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杜仲组培快繁的研究
引用本文:李 琰,张 靖,辛转霞.杜仲组培快繁的研究[J].西北农林科技大学学报(社会科学版),2004,32(6):79-82.
作者姓名:李 琰  张 靖  辛转霞
作者单位:1. 西北农林科技大学,生命科学学院,陕西,杨凌,712100
2. 西北农林科技大学,林学院,陕西,杨凌,712100
3. 渭南市农技推广中心,陕西,渭南,714000
基金项目:国家林业局948项目(99-4-23),西北农林科技大学青年科研专项部分内容
摘    要:研究了植物生长调节剂、基本培养基、采样时间对杜仲腋芽诱导和生长的影响,并对丛芽增殖培养基中植物生长调节剂浓度和生根培养基类型进行了筛选。结果表明,最佳取材时间为4月中旬至6月下旬;以MS为基本培养基,在添加0.05mg/LNAA+0.5mg/LBA或者0.1mg/LIBA+0.5mg/LBA的培养基中,腋芽的诱导和生长较好;继代增殖培养以MS+0.5mg/LBA+0.01mg/LNAA的培养基丛芽数量最多,增殖系数最高;在White培养基上生根率可达57.5%。

关 键 词:杜仲  组织培养  快繁技术
文章编号:1671-9387(2004)06-0079-04
收稿时间:2003/12/24 0:00:00
修稿时间:2003年12月24

Study on tissue culture micropropagation of Eucommia ulmoides Oliv
LI Yan,ZHANG Jing,XIN Zhuan-xia,WANG Ya-jun.Study on tissue culture micropropagation of Eucommia ulmoides Oliv[J].Journal of Northwest Sci-Tech Univ of Agr and,2004,32(6):79-82.
Authors:LI Yan  ZHANG Jing  XIN Zhuan-xia  WANG Ya-jun
Institution:LI Yan~1,ZHANG Jing~2,XIN Zhuan-xia~2,WANG Ya-jun~3
Abstract:The influences of axillary bud induction on growth,plant growth regulator's concentration on multiplication of adventitious bud and basic media on root induction through plant growth regulators were studied,and the basic media,sampling times of Eucommia ulmoides Oliv were selected.The results showed that favorite time for explant collection was from the middle ten days of April to the last ten days of June.MS as the basic medium supplemented with 0.05 mg/L NAA+(0.5 mg/L) BA or 0.1 mg/L IBA+(0.5 mg/L) BA was beneficial for axillary bud induction and growth.The number of adventitious bud and the rate of multiplication were best on MS supplemented with 0.5 mg/L BA +0.01 mg/L NAA.Rooting rate arrived 57.5% on White medium.
Keywords:Eucommia ulmoides Oliv    tissue culture  micropropagation technology
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