| 利用SSR标记鉴定西瓜杂交种纯度的研究 |
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| 引用本文： | 刘子记,詹园凤,朱婕,贺滉,党选民.利用SSR标记鉴定西瓜杂交种纯度的研究[J].热带作物学报,2016,37(9):1714-1718. |
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| 作者姓名： | 刘子记 詹园凤 朱婕 贺滉 党选民 |
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| 作者单位： | 1. 中国热带农业科学院热带作物品种资源研究所 农业部华南作物基因资源与种质创制重点开放实验室 海南儋州571737;2. 海南大学园艺园林学院,海南海口,570228 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金资助项目（No. 1630032014019、1630032015003）。 |
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| 摘 要： | 建立快速、准确、稳定的种子纯度检测技术是保证西瓜杂交种质量的有效措施。利用分子标记技术对小型西瓜‘美月’杂交种纯度进行鉴定。从均匀分布于不同西瓜染色体上的88对SSR引物中筛选出8对引物在‘美月’亲本间表现明显的多态性,分别位于第1、2、3、6、8、9、10染色体上,多态性比率为9.09%,多态性标记均为共显性标记。为了提高检测效率,结合多态性片段大小,同时对标记SSR48和SSR62进行扩增,可成功获得221 bp和131 bp的两组特异性条带,成功建立了双重PCR体系。为了提高鉴定结果的准确性,选用位于西瓜不同染色体上的4对共显性标记对‘美月’进行纯度检测,4对标记的检测结果高度一致,‘美月’杂交种纯度为99.44%。标记鉴定结果与田间表型鉴定结果比较分析表明,2种鉴定结果高度一致。研究结果可为西瓜杂交品种纯度快速检测和品种权保护提供理论依据与技术支撑。
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| 关 键 词： | 小型西瓜 杂交种 双重PCR技术 纯度鉴定 |
Identification of Watermelon Hybrid Purity Using SSR Molecular Marker |
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| LIU Ziji,ZHAN Yuanfeng,ZHU Jie,HE Huang and DANG Xuanmi.Identification of Watermelon Hybrid Purity Using SSR Molecular Marker[J].Chinese Journal of Tropical Crops,2016,37(9):1714-1718. |
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| Authors: | LIU Ziji ZHAN Yuanfeng ZHU Jie HE Huang and DANG Xuanmi |
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| Institution: | Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, P. R. China,;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, P. R. China,;College of Horticulture and Landscape Architecture, Hainan University;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, P. R. China,;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Ministry of Agriculture, P. R. China, |
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| Abstract: | The establishment of purity identification technology with the characteristics of rapid, accurate and stable, is an effective measure to ensure the quality of watermelon hybrid. In this study, molecular marker technology was adopted to evaluate the hybrid purity of mini-watermelon, named Meiyue. Eighty-eight pairs of SSR primers distributing evenly on different watermelon chromosome were used for screening polymorphic markers between the parents of Meiyue. Eight pairs of SSR primers were polymorphic with the rate of 9.09%. All of the polymorphic markers were codominant, distributing on chromosome 1, 2, 3, 6, 8, 9 and 10, respectively. In order to improve the detection efficiency, the SSR48 and SSR62 were amplified simultaneously according to the polymorphic fragments size. Two groups of specific bands with the size of 221 bp and 131 bp were acquired. The duplex PCR technology was successfully constructed. In order to improve the accuracy of the results, 4 pairs of codominant markers on different chromosomes of watermelon were selected to detect the purity of Meiyue. The detection results with the 4 markers were highly consistent. The purity of Meiyue was 99.44%. The results were highly consistent based on molecular marker and phenotypic data. The research results could provide theoretical basis and technical support for the rapid detection of the hybrid purity and the protection of variety right in watermelon. |
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| Keywords: | Mini-watermelon Hybrid Duplex PCR technology Purity identification |
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