| 木霉菌(胞外水解酶)拮抗大豆根腐病病原菌的机制研究 |
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| 引用本文： | 邵红涛,许艳丽.木霉菌(胞外水解酶)拮抗大豆根腐病病原菌的机制研究[J].大豆科学,2006,25(4):429-433. |
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| 作者姓名： | 邵红涛 许艳丽 |
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| 作者单位： | 1. 黑龙江大学农学院,哈尔滨,150080
2. 中国科学院东北地理与农业生态研究所,哈尔滨,150040 |
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| 基金项目： | 黑龙江省重点项目（GB01B201）;黑龙江大学青年教师基金项目（QL200445） |
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| 摘 要： | 通过室内试验与温室试验研究了具有生防能力的木霉菌株Trichoderma MM35所分泌的胞外水解酶在拮抗大豆根腐病病原菌（F．oxysporum、R．solani）中的作用。试验结果表明：以病原菌F．oxysporum烘干的菌丝体作唯一碳源，可以诱导Trichoderma MM35分泌几丁质酶、β-1，3-葡聚糖酶。β-1，3-葡聚糖酶高水平诱导表达在前，几丁质酶诱导表达在后。土壤中接种Trichoderma MM35、F．oxysporum和R．solani之后都能够检测到几丁质酶、β-1，3-葡聚糖酶活性。向有病原菌F．oxysporum的土壤中接种Trichoderma MM35，土壤中几丁质酶活性能够显著升高。向有病原菌R．solani的土壤中接种Trichoderma MM35，土壤中的几丁质酶、G-1，3-葡聚糖酶活性都显著升高。
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| 关 键 词： | 木霉菌 大豆根腐病 机制 胞外酶 几丁质酶 β-1 3-葡聚糖酶 |
| 文章编号： | 1000-9841（2006）04-0429-05 |
| 收稿时间： | 2006-01-04 |
| 修稿时间： | 2006-01-04 |
Assessment of Production of Extracellular Enzymes by Trichoderma spp. For Control of Soybean Root Rot Pathogens (Fusarium oxysporum,Rhizoctonia solani) |
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| Shao Hongtao,Xu Yanli.Assessment of Production of Extracellular Enzymes by Trichoderma spp. For Control of Soybean Root Rot Pathogens (Fusarium oxysporum,Rhizoctonia solani)[J].Soybean Science,2006,25(4):429-433. |
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| Authors: | Shao Hongtao Xu Yanli |
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| Institution: | 1. College of Agriculture, Heilongjiang University, Habin 150080; 2. Northeast Institute of Geography and Agricultural Ecology, Chinese Academy of Sciences, Habin 150040 |
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| Abstract: | The role of extracellular enzymes by Trichoderma MM35 for control of soybean root rot pathogens(Fusarium oxysporum , Rhizoctonia solani) was assessed in vitro and in vivo. Detective levels of hydrolytic extracellular enzymes were recorded by Trichoderma MM35 using dried F. oxysporum mycelium as C-source in vitro or fresh F. oxysporum mycelium or fresh R.solani mycelium in vivo was found that there were significant increases in chitinase activities by Trichoderma MM35 in soil with inoculation of F. oxysporum. Soil infested with Trichoderma MM35 had significantly elevated chitinase and β-1,3-glueanase activities in presence of R. solani as compared to R. solani control. |
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| Keywords: | Trichoderma spp Root rot of soybean Mechanism Extracellular enzyme Chitinase |
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