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中草药提取物对致病性大肠杆菌体外抑菌试验
引用本文:徐峥嵘,薛喜娟,豆思远,贺奋义.中草药提取物对致病性大肠杆菌体外抑菌试验[J].中兽医医药杂志,2011,13(4):53-55.
作者姓名:徐峥嵘  薛喜娟  豆思远  贺奋义
作者单位:甘肃省动物疫病预防控制中心,甘肃兰州,730000
基金项目:甘肃省应用技术研究与开发专项基金
摘    要:根据黄连、黄芩、白头翁、秦皮和穿心莲有效成分的性质,对各药有效成分分别进行提取,获得硫酸黄连素纯品、黄芩苷纯品、30∶1白头翁、30∶1秦皮、20∶1穿心莲提取物,将提取物按一定比例组成配方,采用琼脂平板稀释法,通过单味中草药提取物和组方对仔猪致病性大肠杆菌标准毒株K99和本试验室分离鉴定的O1、O2、O8、O138、O141、078、K89进行体外抑菌试验,结果表明,中药提取物及组方均有较好的抑菌效果,其中组方抑菌效果明显高于单味中草药提取物。

关 键 词:中草药提取物  大肠杆菌  体外抑菌

Bacteriostatic test on extract from traditional Chinese herbs against pathogenic Escherichia coli in vitro
XU Zheng-rong,XUE Xi-juan,DOU Si-yuan,HE Fen-yi.Bacteriostatic test on extract from traditional Chinese herbs against pathogenic Escherichia coli in vitro[J].Journal of Traditional Chinese Veterinary Medicine,2011,13(4):53-55.
Authors:XU Zheng-rong  XUE Xi-juan  DOU Si-yuan  HE Fen-yi
Institution:XU Zheng-rong,XUE Xi-juan,DOU Si-yuan,HE Fen-yi(Gansu Provincial Center for Animal Disease Control and Prevention,Lanzhou Gansu 730000,China)
Abstract:Rhizoma Coptidis,Radix Scutellariae,Radix Pulsatillae,Cortex Fraxini and Herba Andrographitis with low toxicity,antidiarrheal and antipyretic activity were screened by experiments.The efficacious ingredients extracted from them such as purified berberine sulfate,purified baicalin etc.were mixed according to proper ratio.By using the agar plate dilution method,antibacterial test in vitro was conducted with K99,O1,O2,O8,O138,O141,O78 and K89 of pathogenic E.coli on piglets.The results showed both extract from...
Keywords:extract from traditional Chinese herbs  Escherichia coli  bacteriostatic test in vitro  
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