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| 基于matK和rbcL DNA序列条形码鉴定柑橘及其近缘属植物 | |
| 引用本文： | 于杰,闫化学,鲁振华,周志钦.基于matK和rbcL DNA序列条形码鉴定柑橘及其近缘属植物[J].园艺学报,2011,38(9):1733-1740. |
| 作者姓名： | 于杰 闫化学 鲁振华 周志钦 |
| 作者单位： | 1. 南方山地园艺学教育部重点实验室,重庆,400715 2. 中国农业科学院郑州果树研究所,郑州,450009 3. 南方山地园艺学教育部重点实验室,重庆400715／西南大学园艺园林学院,重庆400716 |
| 基金项目： | 国家自然科学基金项目(31171930); 重庆市自然科学基金项目(CSTC2009BB1119); 中央高校基本科研业务费专项(XDJK2011C001); 西南大学科技创新基因项目(SZ201102) |
| 摘 要： | 对柑橘及其近缘属植物59份样品进行叶绿体matK和rbcL的序列测定，序列比对与人工校正，计算属间、种间以及种内的遗传距离，比较序列间的差异，构建系统发育树。结果表明，matK、rbcL及其组合（matK + rbcL）可以对柑橘及其近缘属属间进行鉴定，在种间水平上三者的鉴定率分别为55.9%、37.3%和83.0%。由此可见，与单一片段相比，matK + rbcL序列组合鉴定率更高，可用于对柑橘及其近缘属植物进行物种鉴定。 |
| 关 键 词： | 柑橘属 近缘属 matK rbcL DNA条形码 |
| 收稿时间： | 2011-4-18 |
| 修稿时间： | 2011-8-23 |
Identifying Citrus and Its Related Genera Based on matK and rbcL DNA Sequence as Barcodes |
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| YU Jie,YAN Hua-xue,LU Zhen-hua, ZHOU Zhi-qin,.Identifying Citrus and Its Related Genera Based on matK and rbcL DNA Sequence as Barcodes[J].Acta Horticulturae Sinica,2011,38(9):1733-1740. | |
| Authors: | YU Jie YAN Hua-xue LU Zhen-hua ZHOU Zhi-qin |
| Institution: | YU Jie~1,YAN Hua-xue~1,LU Zhen-hua~3,and ZHOU Zhi-qin~1,2,*(1Key Laboratory of Horticulture Science for Southern Mountainous Regions,Ministry of Education,Chongqing 400715,China,2College of Horticulture and Landscape Architecture,Southwest University,Chongqing 400716,3Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China) |
| Abstract: | The classification of Citrus is still a scientific problem unresolved because of apomixes,asexual variation,and interspecific hybridization in the genus Citrus.To use matK,rbcL,and them together to identify Citrus and its closely related genera,the chloroplast matK and rbcL sequences of 59 accessions were analyzed,the inter-and intraspecific genetic distances were calculated,and the phylogenetic trees of all the accessions tested were constructed based on the obtained distance data.The results indicated tha... |
| Keywords: | Citrus related genera matK rbcL DNA barcode |
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