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| 蜂胶黄酮对宿主细胞抗病毒能力的影响 | |
| 引用本文： | 郭振环,马霞,王恬,沈志强.蜂胶黄酮对宿主细胞抗病毒能力的影响[J].中国畜牧兽医,2016,43(8):2190-2195. |
| 作者姓名： | 郭振环 马霞 王恬 沈志强 |
| 作者单位： | 1. 山东省滨州畜牧兽医研究院, 博士后科研工作站, 滨州 256600; 2. 南京农业大学博士后科研流动站, 南京 210095; 3. 河南省康星药业股份有限公司, 郑州 451464 |
| 基金项目： | 山东省滨州畜牧兽医研究院博士后科研启动基金 |
| 摘 要： | 本试验旨在研究蜂胶黄酮对宿主细胞抗病毒能力的影响。首先采用MTT法测定蜂胶黄酮对宿主鸡胚成纤维细胞（CEF）和PK-15细胞的安全浓度；然后将蜂胶黄酮从最大安全浓度250 μg/mL开始倍比稀释至15.6 μg/mL共5个浓度，将蜂胶黄酮分别与新城疫病毒（NDV）、鸡传染性法氏囊病病毒（IBDV）、猪传染性胃肠炎病毒（TGEV）及猪细小病毒（PPV）一起加到相应长成单层的宿主细胞CEF或PK-15中，在感染后24、48和72 h分别用MTT法测定宿主细胞的存活率，代表蜂胶黄酮提高宿主细胞抗病毒能力的指标。结果显示，蜂胶黄酮可显著提高宿主细胞抗病毒能力，这种活性与其剂量正相关，即剂量越大作用越好。蜂胶黄酮提高宿主细胞抗有囊膜病毒NDV和TGEV作用主要体现在病毒感染的前期，而提高宿主细胞抗无囊膜病毒IBDV和PPV作用主要体现在病毒感染的后期。提示蜂胶黄酮可用于NDV和TGEV的预防及IBDV和PPV的治疗。 |
| 关 键 词： | 蜂胶黄酮 宿主细胞 抗病毒 |
| 收稿时间： | 2016-01-04 |
Effects of Propolis Flavone on Antiviral Ability of Host Cell |
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| GUO Zhen-huan,MA Xia,WANG Tian,SHEN Zhi-qiang.Effects of Propolis Flavone on Antiviral Ability of Host Cell[J].China Animal Husbandry & Veterinary Medicine,2016,43(8):2190-2195. | |
| Authors: | GUO Zhen-huan MA Xia WANG Tian SHEN Zhi-qiang |
| Institution: | 1. Postdoctoral Programme, Binzhou Animal Science and Veterinary Medicine Academy of Shandong Province, Binzhou 256600, China; 2. Postdoctoral Programme, Nanjing Agricultural University, Nanjing 210095, China; 3. Henan Province Health Star Pharmaceutical Co., Ltd., Zhengzhou 451464, China |
| Abstract: | This experiment was conducted to study the effects of propolis flavone (PF) on antiviral ability of host cell.First,the maximal safe concentration of PF to CEF and PK-15 was determined by MTT method.Then PF was diluted to five concentrations (from 250 to 15.6 μg/mL) with maintenance medium and acceded to foster system of CEF or PK-15 with NDV,IBDV,TGEV and PPV,respectively,and the cell viability was observed by MTT method at 24,48 and 72 h after infection as the index of high antiviral ability of host cell.The results showed that PF could promote the antiviral ability of host cell.And the effects were related to PF concentration,as the concentration increased,the effects were better.The antiviral activity of PF to NDV and TGEV with envelope in host cell existed in infectious earlier stage,while for IBDV and PPV without envelope,the activity appeared in infectious later stage.The results indicated that the antiviral effect period and mechanism of propolis flavone was different for different viruses,propolis flavone could be use for the prevention of NDV and TGEV,and for the treatment of IBDV and PPV. |
| Keywords: | propolis flavone host cell antiviral |
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