| 籽鹅和莱茵鹅MSTN和MyoG基因表达及其与屠宰性状的相关分析 |
| |
| 引用本文： | 赵秀华,李满雨,刘国君,许珊珊,孙金艳.籽鹅和莱茵鹅MSTN和MyoG基因表达及其与屠宰性状的相关分析[J].中国畜牧兽医,2016,43(3):738-745. |
| |
| 作者姓名： | 赵秀华 李满雨 刘国君 许珊珊 孙金艳 |
| |
| 作者单位： | 1. 黑龙江省农业科学院博士后科研工作站, 哈尔滨 150086;2. 黑龙江省农业科学院畜牧研究所, 哈尔滨 150086 |
| |
| 基金项目： | 黑龙江省博士后资助项目(LBH-Z13184) |
| |
| 摘 要： | 本研究旨在探讨肌肉生成抑制素(myostatin,MSTN)和肌细胞生成素(myogenin,MyoG)基因对鹅骨骼肌生长发育的影响。试验以莱茵鹅和籽鹅为研究对象,采用实时荧光定量PCR方法测定MSTN、MyoG基因在籽鹅和莱茵鹅胸肌、腿肌中的差异表达情况,运用统计软件对基因表达情况与屠宰性状间的相关性进行分析。结果显示,莱茵鹅胸肌重和胸肌率极显著高于籽鹅(P<0.01),籽鹅胸肌MSTN、MyoG mRNA表达量极显著高于莱茵鹅(P<0.01);在腿肌中,籽鹅MSTN mRNA表达量极显著低于莱茵鹅(P<0.01),籽鹅与莱茵鹅MyoG mRNA表达量间无显著差异(P>0.05)。同一品种MSTN mRNA表达水平也存在差异,在籽鹅胸肌和腿肌中表达量存在极显著差异(P<0.01);在莱茵鹅胸肌和腿肌中表达量存在显著差异(P<0.05)。就MyoG mRNA而言,在籽鹅和莱茵鹅胸肌和腿肌中表达量存在极显著差异(P<0.01)。MSTN基因表达与屠宰性能相关性分析表明,胸肌中MSTN mRNA表达量与活重、胸肌重和胸肌率呈极显著负相关(P<0.01);胸肌和腿肌中MyoG mRNA表达量与活重呈极显著正相关(P<0.01)。说明MSTN和MyoG基因可能对肌肉生长分别起正负调控作用。
|
| 关 键 词： | 鹅 MSTN基因 MyoG基因 mRNA表达 屠宰性能 |
| 收稿时间： | 2015-08-14 |
Expression of MSTN and MyoG Genes in Zi and Rhine Goose and their Correlation with Carcass Traits |
| |
| ZHAO Xiu-hua,LI Man-yu,LIU Guo-jun,XU Shan-shan,SUN Jin-yan.Expression of MSTN and MyoG Genes in Zi and Rhine Goose and their Correlation with Carcass Traits[J].China Animal Husbandry & Veterinary Medicine,2016,43(3):738-745. |
| |
| Authors: | ZHAO Xiu-hua LI Man-yu LIU Guo-jun XU Shan-shan SUN Jin-yan |
| |
| Institution: | 1. Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin 150086, China;2. Animal Husbandry Research Institute, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China |
| |
| Abstract: | The aim of this study was to investigate the effects of MSTN and MyoG genes on goose skeletal muscle growth.In this study,MSTN and MyoG genes expression were detected in breast and leg muscle of Zi and Rhine goose by Real-time PCR,and the correlations between genes expression levels and carcass traits were investigated.The results showed that the breast muscle weight and breast muscle rate of Rhine goose were extremely significant higher than Zi goose (P<0.01).MSTN and MyoG mRNA expression in breast muscle of Zi goose were significantly higher than that of Rhine goose,and the mRNA level of MSTN in leg muscle of Rhine was extremely significant higher than that of Zi goose (P<0.01),there was no significant difference of MyoG mRNA between Zi goose and Rhine goose (P>0.05).There was extremely significant difference between MSTN mRNA expression in breast muscle and leg muscle of Zi goose (P<0.01).MSTN mRNA expression in leg muscle was significantly higher than that of breast muscle of Rhine goose (P<0.05).There was extremely significant difference between MyoG mRNA expression in breast muscle and leg muscle of Zi goose and Rhine goose (P<0.01).There was a extremely significant negative correlation between MSTN mRNA expression in breast muscle with body weight,breast muscle weight and breast muscle percentage (P<0.01).There was a extremely significant positive correlation between MyoG mRNA expression in breast muscle and leg muscle with body weight (P<0.01).MSTN and MyoG gene might have positive and negative regulation effect on muscle growth. |
| |
| Keywords: | goose MSTN gene MyoG gene mRNA expression carcass traits |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
