| 利用Solexa测序技术鉴定策勒黑羊microRNA |
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| 引用本文： | 曾献存,贾斌,史洪才,申红,张永生,陈韩英.利用Solexa测序技术鉴定策勒黑羊microRNA[J].中国畜牧兽医,2016,43(1):204-209. |
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| 作者姓名： | 曾献存 贾斌 史洪才 申红 张永生 陈韩英 |
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| 作者单位： | 1. 石河子大学动物科技学院, 石河子 832003;2. 新疆畜牧科学院农业部家畜繁育重点开发实验室, 乌鲁木齐 832000;3. 石河子大学药学院, 石河子 832003 |
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| 基金项目： | 国家自然科学基金项目(31260536);石河子大学高层次人才启动项目(RCZX201202) |
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| 摘 要： | 试验旨在挖掘、分析策勒黑羊卵巢组织microRNA(miRNA),为进一步研究特定miRNA参与策勒黑羊卵泡发育及激素分泌等相关生殖活动的机制奠定理论基础。从策勒黑羊卵巢组织中提取总RNA,分离、构建小片段RNA文库,进行Solexa测序和生物信息学分析。结果表明,共获得9527311条干净序列读数,小RNA序列长度主要分布在21~23 nt;共鉴定出434个策勒黑羊卵巢组织表达的保守miRNA,其中表达量在100以上有167个;鉴定出57个候选miRNA,其中表达量在100以上有8个。测序成功获取了策勒黑羊卵巢组织miRNA序列及表达谱,卵巢组织miRNA表达丰富且表达量各异。
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| 关 键 词： | miRNA Solexa 卵巢 策勒黑羊 |
| 收稿时间： | 2015-06-30 |
microRNA Identification of Ovaries in Qira Black Sheep by Solexa Sequencing |
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| ZENG Xian-cun,JIA Bin,SHI Hong-cai,SHEN Hong,ZHANG Yong-sheng,CHEN Han-ying.microRNA Identification of Ovaries in Qira Black Sheep by Solexa Sequencing[J].China Animal Husbandry & Veterinary Medicine,2016,43(1):204-209. |
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| Authors: | ZENG Xian-cun JIA Bin SHI Hong-cai SHEN Hong ZHANG Yong-sheng CHEN Han-ying |
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| Institution: | 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;2. Key Laboratory of Ministry for Biotechnology, Xinjiang Academy of Animal Science, Urumqi 832000, China;3. School of Pharmacy, Shihezi University, Shihezi 832003, China |
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| Abstract: | With the purposes of providing basic information for further studies of Qira Black sheep reproduction activities such as folliculogenesis and hormone secretion, characterization of microRNA(miRNA) expression in Qira Black sheep ovary tissues was studied.In this study, the small RNA isolated from total RNA of Qira Black sheep ovary tissues were sequenced by Solexa and then bioinformatics analysis were performed.The results showed that 9527311 clean reads were obtained, and the 21-23 nt small RNA was the major RNA in the total sequences.434 ovary conservative miRNA and 57 new miRNA were identified.Among them, there were 167 conservative including 8 new miRNA exceeded 100 in the expression levels.The study succeeded to construct the expression library of miRNA which were abundant and differentially expressed in Qira Black sheep ovary tissues. |
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| Keywords: | miRNA Solexa ovary Qira Black sheep |
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