| 两种布鲁氏菌弱毒株侵染小鼠巨噬细胞过程的荧光表征及分析 |
| |
| 引用本文： | 胡梦薇,刘朋涛,严国,高剑峰.两种布鲁氏菌弱毒株侵染小鼠巨噬细胞过程的荧光表征及分析[J].中国畜牧兽医,2016,43(8):1944-1950. |
| |
| 作者姓名： | 胡梦薇 刘朋涛 严国 高剑峰 |
| |
| 作者单位： | 石河子大学生命科学学院, 石河子 832003 |
| |
| 基金项目： | 国家重点基础研究发展计划（973计划）（2010CB530200） |
| |
| 摘 要： | 通过对绿色荧光蛋白（GFP）布鲁氏菌弱菌株M5（GFP-M5）和S19（GFP-S19）侵染小鼠巨噬细胞（RAW264.7）及对其与胞内溶酶体、内质网、高尔基体初次结合所用时间进行测定，探讨分析两种布鲁氏菌弱毒株侵染小鼠巨噬细胞过程的荧光表征。将GFP-M5和GFP-S19分不同时间段分别侵染RAW264.7，利用激光共聚焦和流式细胞仪观察和检测。结果显示，GFP-M5和GFP-S19均构建成功。布鲁氏菌M5和S19及GFP-M5和GFP-S19侵染RAW264.7后胞内生存能力无明显差异。GFP-M5和GFP-S19侵染30 min后均已进入小鼠巨噬细胞，2 h分别到达溶酶体、内质网和高尔基体。而两种弱毒株在1、2、3、4 h与各细胞器结合率相近。流式细胞仪检测结果显示，GFP-M5和GFP-S19侵染RAW264.7的GFP+细胞含量无显著差异（P>0.05）。结果提示，两种弱毒株在侵染进入宿主细胞的初期及侵袭能力并没有明显差异。
|
| 关 键 词： | 布鲁氏菌 绿色荧光蛋白(GFP) RAW264.7 共聚焦显微镜 流式细胞仪 |
| 收稿时间： | 2016-01-21 |
The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte |
| |
| HU Meng-wei,LIU Peng-tao,YAN Guo,GAO Jian-feng.The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte[J].China Animal Husbandry & Veterinary Medicine,2016,43(8):1944-1950. |
| |
| Authors: | HU Meng-wei LIU Peng-tao YAN Guo GAO Jian-feng |
| |
| Institution: | College of Life Science, Shihezi University, Shihezi 832003, China |
| |
| Abstract: | The experiment was conducted to discuss the difference of binding time of green fluorescent protein B.melitensis M5 (GFP-M5) and B.abortus S19 (GFP-S19) infecting the mouse macrophagocyte (RAW264.7),lysosome,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP-M5,GFP-S19 with organelle in different timeline,respectively,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP-M5 and GFP-S19 were successfully constructed.The intracellular survival ability of Brucella M5,Brucella S19,GFP-M5 and GFP-S19 were not obvisouly affected after infecting RAW264.7.GFP-M5 and GFP-S19 could enter the macrophagocyte in 30 mins,and in 2 h the Brucella could reach lysosome,endoplasmic reticulum and golgi body.In addition,the binding time for two attenuated vaccine did not show differences in 1,2,3 and 4 h.The content of GFP+ cell produced by RAW264.7 infected by GFP-M5 and GFP-S19 did not show significant differences (P>0.05).Therefore,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell. |
| |
| Keywords: | Brucella green fluorescent protein RAW264 7 confocal laser scanning microscope flow cytometry |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
