| 山羊呼吸系统疾病病原分析 |
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| 引用本文： | 陶立,蓝显利,李军,马春霞,蓝金红,黄明学,兰美益,闭炳芬,陈泽祥,杨威.山羊呼吸系统疾病病原分析[J].中国畜牧兽医,2016,43(4):1072-1078. |
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| 作者姓名： | 陶立 蓝显利 李军 马春霞 蓝金红 黄明学 兰美益 闭炳芬 陈泽祥 杨威 |
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| 作者单位： | 1. 广西兽医研究所, 南宁 530001;2. 广西畜禽疫苗新技术重点实验室, 南宁 530001;3. 广西马山县动物疫病预防控制中心, 马山 530600 |
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| 基金项目： | 南宁市科技攻关项目(20132312);广西壮族自治区水产畜牧局科研项目(桂渔牧科201528002、桂渔牧科08283-2) |
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| 摘 要： | 为了调查广西省某山羊场一起羊呼吸道疾病的病原,本试验采取流行病学调查、临床症状与病理变化观察、病原分离鉴定、生化试验、PCR及致病性试验等方法对病原进行分析,并根据流行病学特点和药敏试验结果进行防控。结果表明,从病死山羊的肺脏组织中分离出MS1和MS2两株革兰氏阴性的致病杆菌,分离培养或PCR检测发现支原体、流感病毒和寄生虫均为阴性。MS1菌株生化特性符合黏质沙雷氏菌,其16S rRNA基因测序结果与GenBank上登录的黏质沙雷氏菌核苷酸序列同源性达到99%以上;MS2菌株生化特性符合大肠杆菌,其16S rRNA基因测序结果与GenBank上登录的大肠杆菌核苷酸序列同源性达到99%以上,两株菌对小白鼠均具有致病性。大肠杆菌和黏质沙雷氏菌均对壮观霉素、阿米卡星、卡那霉素和新霉素高度敏感,用高敏药物卡那霉素联合地塞米松等相关措施进行治疗,收到良好效果。
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| 关 键 词： | 山羊呼吸道疾病 病原分析 黏质沙雷氏菌 大肠杆菌 |
| 收稿时间： | 2015-09-16 |
Pathogen Analysis of Goat's Respiratory Diseases |
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| TAO Li,LAN Xian-li,LI Jun,MA Chun-xia,LAN Jin-hong,HUANG Ming-xue,LAN Mei-yi,BI Bing-fen,CHEN Ze-xiang,YANG Wei.Pathogen Analysis of Goat's Respiratory Diseases[J].China Animal Husbandry & Veterinary Medicine,2016,43(4):1072-1078. |
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| Authors: | TAO Li LAN Xian-li LI Jun MA Chun-xia LAN Jin-hong HUANG Ming-xue LAN Mei-yi BI Bing-fen CHEN Ze-xiang YANG Wei |
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| Institution: | 1. Guangxi Veterinary Research Institute, Nanning 530001, China;2. Guangxi Key Laboratory of Animal Vaccines and New Technology, Nanning 530001, China;3. Animal Disease Prevention and Control Center of Mashan, Mashan 530600, China |
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| Abstract: | To investigate the pathogens of goat's respiratory disease from a goat farm in Guangxi province, the epidemiological investigation, clinic observation, pathological examination, bacterium isolation and identification, biochemical test, PCR and animal experiment test were conducted.The preventive and control measures were taken on the basis of pathogen epidemiological characteristics and drug sensitive test results.The results showed that Mycoplasma, influenza virus and parasite were negative by isolation and culture or PCR, however, two strains of gram-negative bacteria named MS1 and MS2 were isolated from lung.MS1 was identified as Serratia marcescens by biochemical test and 16S rRNA sequencing, which shared 99% nucleotide homology with other Serratia marcescens in GenBank.And MS2 was identified as E.coil by the same methods that shared 99% nucleotide homology with other E.coli in GenBank.Animal experiment showed that two strains could cause death in mice.The drug sensitive tests showed two strains were highly sensitive to spectinomycin, amikacin, kanamicin and neomycin.Kanamycin and dexamethasone were used to treat the sick goats, and achieved good results. |
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| Keywords: | goat's respiratory diseases pathogen analysis Serratia marcescens E coli |
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