| 伪狂犬病病毒变异株的鉴定及伪狂犬病的防控 |
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| 引用本文： | 黄元,陈晶,赵翠玲,陈锦良,向蓉,王晓虎,向华,黄忠.伪狂犬病病毒变异株的鉴定及伪狂犬病的防控[J].中国畜牧兽医,2016,43(9):2461-2467. |
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| 作者姓名： | 黄元 陈晶 赵翠玲 陈锦良 向蓉 王晓虎 向华 黄忠 |
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| 作者单位： | 广东省农业科学院动物卫生研究所, 广东省兽医公共卫生公共实验室, 广东省畜禽疫病防治研究重点实验室, 广州 510640 |
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| 基金项目： | 广东省科技计划项目（2015A020224026、2015A020208007）；广东省农业科学院院长基金项目（201628） |
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| 摘 要： | 2011年以来伪狂犬病病毒（PRV）变异株在中国大范围流行致伪狂犬病（PR）再次暴发。广东某猪场发生疑似PR引起母猪较大范围的流产，为此本试验展开对该病诊断和防控方法的研究。随机抽取流产和未流产母猪血清，应用ELISA检测PRV gE和gB抗体；同时采集发病仔猪脑组织PCR检测PRV gH片段。对全场母猪紧急接种PRV变异株灭活苗，分别应用ELISA和中和试验检测免疫前后的血清抗体。结果显示，已发生流产母猪血清PR gE抗体均为阳性，而未流产母猪血清抗体见弱阳性；流产母猪PRV gB抗体的S/P值高达4.0，未流产母猪也达3.3。PCR检测3头病仔的脑组织均为阳性，测序表明其gB基因与2012年流行毒株BJ-YT-2012序列相似性为100%。ELISA检测免疫灭活疫苗前母猪血清PRV gB抗体S/P值为1.603，免疫4周后升高到2.88；特别是中和抗体从1：24升高到1：213。这与免疫疫苗1周后母猪流产开始减少，2周后母猪少见流产的结果吻合。研究结果提示，PRV经典株疫苗产生的PRV gB抗体对变异株的保护作用不佳，而变异株疫苗的保护效果显著。
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| 关 键 词： | 伪狂犬病病毒 变异株 检测 鉴定 免疫 |
| 收稿时间： | 2016-03-01 |
Identification of Pseudorabies Virus Variant and Control of Pseudorabies |
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| HUANG Yuan,CHEN Jing,ZHAO Cui-ling,CHEN Jin-liang,XIANG Rong,WANG Xiao-hu,XIANG Hua,HUANG Zhong.Identification of Pseudorabies Virus Variant and Control of Pseudorabies[J].China Animal Husbandry & Veterinary Medicine,2016,43(9):2461-2467. |
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| Authors: | HUANG Yuan CHEN Jing ZHAO Cui-ling CHEN Jin-liang XIANG Rong WANG Xiao-hu XIANG Hua HUANG Zhong |
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| Institution: | Guangdong Provincial Key Laboratory of Livestock Disease Prevention, Veterinary Public Health Laboratory of Guangdong Province, Institute of Animal Health, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China |
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| Abstract: | Due to variant strain,pseudorabies virus (PRV) has broken out again and spread in China since 2011.A swine farm in Guangdong province was found pseudorabies (PR) symptoms-like miscarriage after introduction.The study was carried out to identify and control the PR.Serum of sows with and without miscarriage were randomly collected and the PRV gB and gE were detected by ELISA method,and brain tissues of sick piglets were sampled and the PRV gH gene was tested by PCR.All the sows in the farm were emergently inoculated PRV variant strainin activated vaccine.Serum before and after immunization were collected and detected by ELISA and micro-serum neutralization test.ELISA results showed that gE antibody of all the breeding sows with miscarriage were positive,and that of sows without miscarriage showed weekly positive;The average gB ELISA S/P value of sows with miscarriage was as high as 4.0,while that of sows without miscarriage was over 3.0.PCR of 3 sick piglets were all positive and the sequence of gB gene was 100% identical to BJ-YT-2012,a wide variant stain in 2012. The result of detection of the sows serum at before and after immunization showed that the S/P value of gB rose up from 1.603 before immunization to 2.88 at four weeks after immunization,and the neutralizing antibody rose up from 1:24 to 1:213.This agreed with the results that the sows showed less probability of miscarriage since the first week after immunization and almost no miscarriage after two weeks after immunization.This study suggested that classical PRV vaccine was not effective in this case,while the vaccine made from the variant PRV strain was. |
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| Keywords: | pseudorabies virus variant detection identification immunization |
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