| 黑羽鹌鹑的EST-SSR标记开发与应用 |
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| 引用本文： | 白俊艳,庞有志,杨又兵,祁艳霞,李宏伟.黑羽鹌鹑的EST-SSR标记开发与应用[J].中国畜牧兽医,2016,43(5):1323-1328. |
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| 作者姓名： | 白俊艳 庞有志 杨又兵 祁艳霞 李宏伟 |
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| 作者单位： | 河南科技大学动物科技学院, 洛阳 471003 |
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| 基金项目： | 河南省科技厅项目(2015HNCXY012) |
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| 摘 要： | 本研究旨在加速分子标记在黑羽鹌鹑遗传育种等方面的应用,开发黑羽鹌鹑的EST-SSR标记。随机选取100只黑羽鹌鹑,心脏采血2mL,采用生物信息学方法开发黑羽鹌鹑的EST-SSR新标记,并检测EST-SSR标记在黑羽鹌鹑群体中的多态性。结果显示,在黑羽鹌鹑中成功的开发出了6个EST-SSR标记,EST-SSR标记检测到的观测等位基因数为2~6个,6个EST-SSR标记在黑羽鹌鹑的平均多态信息含量(PIC)为0.4962,平均期望杂合度(He)为0.5759。6个EST-SSR标记中P1和P2个为高度多态标记,其他4个EST-SSR为中度多态标记。新开发的6个EST-SSR标记可用于黑羽鹌鹑的遗传多样性分析,6个EST-SSR标记与鹌鹑的糖类代谢、黑色素的合成等有关系,但具体的机理还有待进一步分析。
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| 关 键 词： | 黑羽鹌鹑 EST-SSR 生物信息学 遗传多样性 |
| 收稿时间： | 2015-09-28 |
Development and Application of EST-SSR Markers of Black Quail |
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| BAI Jun-yan,PANG You-zhi,YANG You-bing,QI Yan-xia,LI Hong-wei.Development and Application of EST-SSR Markers of Black Quail[J].China Animal Husbandry & Veterinary Medicine,2016,43(5):1323-1328. |
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| Authors: | BAI Jun-yan PANG You-zhi YANG You-bing QI Yan-xia LI Hong-wei |
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| Institution: | College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471003, China |
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| Abstract: | The purpose of this study was to accelerate the application of molecular markers in genetic breeding and other aspects of Black quail to develop EST-SSR markers for Black quail.100 Black quail were randomly chosen and blood samples were collected to develop EST-SSR new markers and determined the genetic polymorphism using bioinformatics.The results showed that there were 6 EST-SSR markers in the Black quail, the number of observed alleles in EST-SSR was 2 to 6.The average PIC of 6 EST-SSR markers in the Back quail was 0.4962 and the average He was 0.5759.Markers P1 and P2 were highly polymorphic markers among 6 EST-SSR and the rest EST-SSR markers were moderately polymorphic markers.These developed markers could be used for genetic diversity analysis of Black quail and the EST-SSR markers had some relationship with the carbohydrate metabolism, synthesis of melanin in Black quail, but the mechanism needed the further analysis. |
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| Keywords: | Black quail EST-SSR bioinformatics genetic diversity |
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