| 1株H6N6亚型禽流感病毒贵州分离株HA和NA基因克隆与序列分析 |
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| 引用本文： | 陈佳琪,嵇辛勤,段志强,文明,阮涌,雷云,侯萍.1株H6N6亚型禽流感病毒贵州分离株HA和NA基因克隆与序列分析[J].中国畜牧兽医,2016,43(2):296-303. |
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| 作者姓名： | 陈佳琪 嵇辛勤 段志强 文明 阮涌 雷云 侯萍 |
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| 作者单位： | 1. 贵州大学动物科学学院, 贵阳 550025;2. 贵州省动物疫病研究室, 贵阳 550025;3. 贵州省种畜禽种质测定中心, 贵州贵阳 550025 |
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| 基金项目： | 贵州省规模养鸭场病原菌耐药性调查与控制研究(黔科合NY[2013]3074号);水禽免疫增强剂创制与开发关键技术研究(黔科合计省合[2013]7004号) |
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| 摘 要： | 为了解 H6亚型禽流感病毒(AIV)在贵州地区的流行情况,本研究对2014年从贵州省三穗鸭体内分离鉴定出的1株H6N6亚型AIV (A/duck/Guizhou/013/2014) HA和NA基因进行了克隆和序列分析。结果显示,A/duck/Guizhou/013/2014的HA基因与华东地区2009年鸭源H6N6亚型AIV同源性最高,达97.5%,HA蛋白裂解位点的氨基酸序列为P-Q-I-E-T-R-G,符合低致病性AIV的分子特征;而NA基因则与福建2007年鸭源H6N6亚型AIV同源性最高,达98.2%;由遗传进化树分析结果可知,HA和NA基因在遗传进化关系上,与湖南毒株位于同一分支,而与2007年贵州分离的3株H6N6亚型AIV不处于同一分支,说明A/duck/Guizhou/013/2014与本地区的H6N6亚型AIV亲缘关系较远。本研究结果表明当前贵州地区H6N6亚型AIV存在明显的遗传多样性。
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| 关 键 词： | 禽流感病毒 H6N6亚型 血凝素基因 神经氨酸酶基因 序列分析 |
| 收稿时间： | 2015-07-21 |
Cloning and Sequence Analysis of HA and NA Genes of One Strain of H6N6 Subtype Avian Influenza Virus Isolated from Guizhou Province |
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| CHEN Jia-qi,JI Xin-qin,DUAN Zhi-qiang,WEN Ming,RUAN Yong,LEI Yun,HOU Ping.Cloning and Sequence Analysis of HA and NA Genes of One Strain of H6N6 Subtype Avian Influenza Virus Isolated from Guizhou Province[J].China Animal Husbandry & Veterinary Medicine,2016,43(2):296-303. |
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| Authors: | CHEN Jia-qi JI Xin-qin DUAN Zhi-qiang WEN Ming RUAN Yong LEI Yun HOU Ping |
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| Institution: | 1. College of Animal Science, Guizhou University, Guiyang 550025, China;2. Laboratory for Animal Epidemic Disease of Guizhou, Guiyang 550025, China;3.Kind of livestock and poultry species determination center of Guizhou, Guiyang 550025, China |
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| Abstract: | To investigate the epidemic situation of H6N6 subtype avian influenza virus (AIV) in Guizhou province,A/duck/Guizhou/013/2014 was isolated from Sansui duck in live poultry market of Guizhou in 2014,the hemagglutinin (HA) and neuraminidase (NA) genes of DK/GZ/14 were subjected to clone and sequence analysis.The results showed that HA gene had the highest nucleotide homologies (97.5%) with the duck-origin H6N6 subtype AIV isolated from Eastern China in 2009,and the strains of HA gene proteolytic cleavage sites was P-Q-I-E-T-R-G,which accordeol with the molecular characteristic of low pathogenic AIV (LPAIV).However,NA gene of A/duck/Guizhou/013/2014 had the highest nucleotide homologies (98.2%) with the duck-origin H6N6 subtype AIV isolated from Fujian in 2007.The phylogenetic tree showed that A/duck/Guizhou/013/2014 and Hunan strains located in the same branch,while three duck-origin H6N6 subtype AIV isolated from Guizhou in 2007 and A/duck/Guizhou/013/2014 located in the different branch for HA and NA genes in genetic evolution,which suggested that A/duck/Guizhou/013/2014 was far with the local H6N6 subtype.The results also clearly indicated that duck-origin H6N6 subtype AIV had genetic diversity in duck population in Guizhou. |
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| Keywords: | avian influenza virus H6N6 subtype HA gene NA gene sequence analysis |
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