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| 奶牛乳房炎白色念珠菌的分离鉴定及生物学特性研究 | |
| 引用本文： | 何芳,卢金霞,冯峰,周学章.奶牛乳房炎白色念珠菌的分离鉴定及生物学特性研究[J].中国畜牧兽医,2016,43(12):3356-3362. |
| 作者姓名： | 何芳 卢金霞 冯峰 周学章 |
| 作者单位： | 1. 宁夏大学西部特色生物资源保护与利用教育部重点实验室, 银川 750021; 2. 宁夏智弘生物科技有限公司, 银川 750021 |
| 基金项目： | 国家自然科学基金项目（31660728） |
| 摘 要： | 试验旨在了解宁夏地区奶牛真菌性乳房炎的发生情况，为诊断和治疗真菌性奶牛乳房炎提供有效依据。采集宁夏周边地区各奶牛场使用抗生素治疗无效的乳房炎奶样，对奶样中的真菌进行分离鉴定，并对分离菌进行生物学特性研究。通过选择性培养基从乳房炎奶样中获得真菌，进一步应用生物化学和分子生物学手段鉴定出样品中的真菌为白色念珠菌。通过溶血性、磷脂酶活性及产膜等方面的研究，证实其具有不同毒力，分离菌株对试验动物有较强的致病性。同时，采用药敏纸片法对分离株进行药敏试验，结果显示，菌株对临床常用抗生素耐药，对临床常用抗真菌药物敏感。结果表明，宁夏地区真菌性乳房炎奶样中分离出的致病菌均为白色念珠菌，感染率为43%，分离株具有较强的毒力，对常用抗生素耐药，对抗真菌药物和中药敏感。 |
| 关 键 词： | 白色念珠菌 乳房炎 致病性 溶血性 |
| 收稿时间： | 2016-05-16 |
Isolation,Identification and Analysis of Biological Characteristics of Candida albicans in Bouine Mastitis |
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| HE Fang,LU Jin-xia,FENG Feng,ZHOU Xue-zhang.Isolation,Identification and Analysis of Biological Characteristics of Candida albicans in Bouine Mastitis[J].China Animal Husbandry & Veterinary Medicine,2016,43(12):3356-3362. | |
| Authors: | HE Fang LU Jin-xia FENG Feng ZHOU Xue-zhang |
| Institution: | 1. Key Laboratory of Ministry of Education for Protection and Utilization of Special Biological Resources in Western China, Ningxia University, Yinchuan 750021, China; 2. Ningxia Zoohance Biotech Co., Ltd., Yinchuan 750021, China |
| Abstract: | The study was aimed to learn the infection of dairy cows with fungi in Ningxia and take effective measures to prevent and control the fungi infection. Milk samples were collected from dairy cows that did not respond to antibiotics in Ningxia, fungi in milk samples were separated and identified, and the biological characteristics of isolated bacteria were analyzed. Milk samples infected by fungus were screened out using fungal selective medium. The yeast pathogen was shown to be Candida albicans by biochemistry and molecular biology methods. Determination of enzyme activity and biofilm-forming tests confirmed that it had different levels of virulence with strong pathogenicity to experimental animals. Drug sensitive test was carried out by K-B programe, the results showed that the isolates were resistant to clinical commonly used antibiotics, and were sensitive to clinical common antifungal drugs. The fungal mastitis was caused by Candida albicans in Ningxia, the ratio of fungal infection was 43%. Candida albicans had strong virulence and were resistant to common antibiotic and sensitivity to antifungal drugs and traditional Chinese medicine. |
| Keywords: | Candida albicans mastitis pathogenicity haemolysin |
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