| 蓖麻蛋白对人外周血B淋巴细胞的作用及相关基因表达的研究 |
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| 引用本文： | 甄二东,黄素娟,化朝举,陶聪,杨述林,王彦芳,周荣,敖红,李奎.蓖麻蛋白对人外周血B淋巴细胞的作用及相关基因表达的研究[J].中国畜牧兽医,2016,43(11):2803-2810. |
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| 作者姓名： | 甄二东 黄素娟 化朝举 陶聪 杨述林 王彦芳 周荣 敖红 李奎 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所, 农业部畜禽遗传资源与种质创新重点实验室, 北京 100193 |
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| 基金项目： | 转基因新品种培育重大专项（2014ZX08011-006）；中国农业科学院科技创新工程（ASTIP-IAS05） |
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| 摘 要： | 试验旨在研究蓖麻蛋白对人外周血B淋巴细胞（IM-9）毒性作用及对相关基因表达的影响。将提取的蓖麻蛋白按不同浓度添加到培养基培养6、12 h，研究剂量－时间效应，确定半数抑制浓度，然后以半数抑制浓度培养细胞2、6、10、12 h，在每个时间段检测免疫相关基因CD40、IL-1β、TNF-α和凋亡相关基因Bcl-2、Bax、Caspase-3的表达情况。结果表明，蓖麻蛋白对IM-9细胞的毒性作用随浓度和时间的增加而增强。取接近半数抑制浓度20 ng/mL作用IM-9细胞2、6、10、12 h，免疫相关基因中，TNF-α和CD40基因在6 h试验组表达量与对照组相比显著升高（P<0.05），10、12 h达到极显著水平（P<0.01）；IL-1β基因表达量试验组、对照组间差异不显著（P>0.05）。凋亡基因中，与对照比相比，试验组Bax基因表达量10 h极显著降低（P<0.01），12 h显著降低（P<0.05）；Bcl-2基因表达量4个时间段均达到显著水平（P<0.05），2、12 h达到极显著水平（P<0.01）；Caspase-3基因除6 h表达量降低外，其他时间段表达量均显著升高（P<0.05），2 h为极显著水平（P<0.01）。表明蓖麻蛋白能显著影响IM-9细胞的基因表达，TNF-α和CD40基因是两个潜在用IM-9细胞评价蛋白毒性的检测基因。
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| 关 键 词： | 蓖麻蛋白 外周血B淋巴细胞 基因表达 |
| 收稿时间： | 2016-04-25 |
Study on the Effect of Ricin on Human Peripheral Blood B Lymphocyte and the Expression of Related Genes |
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| ZHEN Er-dong,HUANG Su-juan,HUA Chao-ju,TAO Cong,YANG Shu-lin,WANG Yan-fang,ZHOU Rong,AO Hong,LI Kui.Study on the Effect of Ricin on Human Peripheral Blood B Lymphocyte and the Expression of Related Genes[J].China Animal Husbandry & Veterinary Medicine,2016,43(11):2803-2810. |
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| Authors: | ZHEN Er-dong HUANG Su-juan HUA Chao-ju TAO Cong YANG Shu-lin WANG Yan-fang ZHOU Rong AO Hong LI Kui |
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| Institution: | Key Laboratory of Farm Animal Genetic Resources and Germplam Innovation, Ministry of Agriculture, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | This study was amied to learn the toxic effects of ricin on human peripheral blood B lymphocytes（IM-9）and the expression of related genes.The extracted ricin of different concentrations were added to culture cells for 6 and 12 h to make sure the median inhibitory concentration,then culture cells at the median inhibitory concentration for 2,6,10 and 12 h,at each time point detect the expression of immune related genes CD40,IL-1β,TNF-α and apoptosis related genes Bcl-2,Bax,Caspase-3.The results showed that the inhibitory effect of ricin on IM-9 cells was increased with the increase of concentration and time.The expression of TNF-α and CD40 genes increased significantly in experimental group than control group at 6 h（P<0.05).10 and 12 h reached extremely significant level（P<0.01);There was no significance of IL-1β gene expression between experimental and control groups（P>0.05).About apoptosis gene,the Bax gene expression decreased extremely significantly in experimental group at 10 h（P<0.05）and decreased significantly at 12 h（P<0.05);Bcl-2 gene had reached significant levels of four time periods（P<0.05), and 2,12 h were extremely significant（P<0.01);The expression of Caspase-3 reduced at 6 h,the other time points increased significantly（P<0.05),at 2 h reached extremely significant level（P<0.01).It showed that the gene expression of IM-9 cells could be significantly affected by ricin,TNF-α and CD40 genes were two potential genes to evaluate the toxicity by IM-9 cells. |
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| Keywords: | ricin peripheral blood B lymphocyte gene expression |
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