| 绵羊CLPG和MSTN基因多态性及其与生长性状的关联分析 |
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| 引用本文： | 胡师金,张淑珍,袁泽湖,轩俊丽,马晓萌,张莉,赵福平,魏彩虹,杜立新.绵羊CLPG和MSTN基因多态性及其与生长性状的关联分析[J].中国畜牧兽医,2016,43(5):1285-1293. |
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| 作者姓名： | 胡师金 张淑珍 袁泽湖 轩俊丽 马晓萌 张莉 赵福平 魏彩虹 杜立新 |
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| 作者单位： | 中国农业科学院北京畜牧兽医研究所, 北京 100193 |
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| 基金项目： | 国家科技支撑计划项目(2011BAD28B05-2);国家肉羊产业技术体系(ASTIP-IAS-TS-6) |
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| 摘 要： | 试验旨在探究CLPG(Callipyge)与MSTN(Myostatin)基因作为绵羊生长性状候选基因的可能性,调查与绵羊生长性状相关的分子遗传标记。本试验以133只澳洲白羊×杜泊羊×湖羊杂交绵羊为研究对象,利用PCR产物直接测序及PCR-RFLP技术检测CLPG与MSTN基因的单核苷酸多态性,然后通过SPSS22.0软件GLM统计模型分析多态位点不同基因型及聚合基因型与绵羊生长性状的关联性。测序结果表明,CLPG基因STS序列232bp处检测到C→T突变位点C1,MSTN基因3'UTR区检测到G→A突变位点M1。PCR-RFLP分析显示,C1位点表现为2种基因型:CC和CT;M1位点表现为2种基因型:GG和GA。关联分析表明,C1位点与绵羊背膘厚和眼肌面积显著或极显著相关(P<0.05;P<0.01),M1位点与绵羊体重、管围、背膘厚和眼肌面积显著或极显著相关(P<0.05;P<0.01)。聚合基因型对体重、背膘厚和眼肌面积影响极显著(P<0.01)。研究揭示CLPG与MSTN基因多态性及其聚合基因型对绵羊生长性状具有显著影响,C1和M1位点可以考虑作为绵羊生长性状的有效遗传标记。
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| 关 键 词： | CLPG基因 MSTN基因 多态性 生长性状 关联分析 |
| 收稿时间： | 2015-11-13 |
Polymorphism of CLPG and MSTN Genes and their Association Analysis with Growth Traits in Sheep |
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| HU Shi-jin,ZHANG Shu-zhen,YUAN Ze-hu,XUAN Jun-li,MA Xiao-meng,ZHANG Li,ZHAO Fu-ping,WEI Cai-hong,DU Li-xin.Polymorphism of CLPG and MSTN Genes and their Association Analysis with Growth Traits in Sheep[J].China Animal Husbandry & Veterinary Medicine,2016,43(5):1285-1293. |
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| Authors: | HU Shi-jin ZHANG Shu-zhen YUAN Ze-hu XUAN Jun-li MA Xiao-meng ZHANG Li ZHAO Fu-ping WEI Cai-hong DU Li-xin |
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| Institution: | Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China |
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| Abstract: | The study was conducted to explore the possibility that CLPG (Callipyge) and MSTN (Myostatin) genes which could be the candidate genes of sheep growth traits, and investigate the molecular genetic markers related to sheep growth traits.133 (Austrilian White sheep×Dorper sheep×Hu sheep) hybid-sheep were chosen as subjects, the technology of direct sequencing of PCR products and PCR-RFLP were used to detect the single nucleotide polymorphism of CLPG and MSTN genes, then the association of the SNPs different genotypes and combined genotypes with sheep growth traits were analyzed by the GLM statistical model of SPSS 22.0.Sequencing results showed that the SNP of C/T which called C1 was detected at position 232 bp of the STS sequence in CLPG gene.The SNP of G/A which called M1 was detected in the 3'UTR of MSTN gene.PCR-RFLP analysis showed that two genotypes CC and CT were in C1 site, two genotypes GG and GA were in M1 site.Association analysis revealed that C1 site was significantly or extremely significantly associated with backfat thickness and loin muscle area (P<0.05;P<0.01), M1 site was significantly or extremely significantly associated with body weight, tube girth, backfat thickness and loin muscle area (P<0.05;P<0.01).Meanwhile, the combined genotype was extremely significantly associated with body weight, backfat thickness and loin muscle area (P<0.01).The conclusions indicated that SNPs and combined genotype of CLPG and MSTN genes had effects on growth traits in sheep.C1 and M1 sites could be considered as effective genetic markers for sheep growth traits. |
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| Keywords: | CLPG gene MSTN gene polymorphism growth traits association analysis |
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