| 5个猪品种3个拷贝数变异区的多态性分析 |
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| 引用本文： | 黄世会,冉雪琴,王嘉福,潘华,李蓉蓉.5个猪品种3个拷贝数变异区的多态性分析[J].中国畜牧兽医,2016,43(3):730-737. |
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| 作者姓名： | 黄世会 冉雪琴 王嘉福 潘华 李蓉蓉 |
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| 作者单位： | 1. 贵州大学动物科学学院, 贵阳 550025;2. 贵州大学农业生物工程研究院, 贵阳 550025 |
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| 基金项目： | 国家高技术研究发展计划(863计划)课题(2013AA102503);贵州省农业科技攻关项目(黔科合NY[2013]3073号);贵州省科技创新人才团队建设专项(黔科合人才团队2009-4006);贵州大学"本科教学工程"项目(BKJX2012007) |
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| 摘 要： | 为了探究拷贝数变异(copy number variation,CNV)在不同猪品种间的多态性,本试验根据猪SNP60芯片检测结果,以大白猪、香猪、柯乐猪、糯谷猪、荣昌猪为主要研究对象,采用实时荧光定量PCR方法,对5个猪品种基因组中3个CNV区域(CNVR91、CNVR92和CNVR143)的拷贝数进行测定。结果显示,香猪的CNVR91以拷贝数缺失为主,其他4个猪品种的拷贝数以正常为主;香猪、大白猪、柯乐猪、荣昌猪的CNVR92以拷贝数缺失为主,糯谷猪以拷贝数正常为主;香猪和糯谷猪的CNVR143以拷贝数增加为主,其他3个猪品种以拷贝数正常为主。表明3个拷贝数变异区在5个猪品种之间具有多态性,可能通过剂量效应影响香猪、糯谷猪和柯乐猪等相关基因的表达和生理功能。
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| 关 键 词： | 拷贝数变异 猪品种 实时荧光定量PCR 多态性 |
| 收稿时间： | 2015-11-17 |
Analysis on Polymorphisms of Three CNV Regions in Five Pig Breeds |
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| HUANG Shi-hui,RAN Xue-qin,WANG Jia-fu,PAN Hua,LI Rong-rong.Analysis on Polymorphisms of Three CNV Regions in Five Pig Breeds[J].China Animal Husbandry & Veterinary Medicine,2016,43(3):730-737. |
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| Authors: | HUANG Shi-hui RAN Xue-qin WANG Jia-fu PAN Hua LI Rong-rong |
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| Institution: | 1. College of Animal Science, Guizhou University, Guiyang 550025, China;2. Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China |
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| Abstract: | The paper was aimed to investigate the polymorphism of copy number variation (CNV) in different pig breeds.Three CNV regions of CNVR91,CNVR92 and CNVR143 were chosen from the porcine SNP60 chip genotyping results.The polymorphisms of three CNVs were determined by Real-time quantitative PCR method,taking five pig breeds as samples,including Yorkshire pig,Xiang pig,Kele pig,Nuogu pig and Rongchang pig breeds.The results showed that the dominant status of CNVR91 was loss in Xiang pig,while it was normal in other four pig breeds.The major type of CNVR92 was deletion in Xiang pig,Yorkshire pig,Kele pig and Rongchang pig breeds,with a high normal percent in Nuogu pig.For CNVR143,the dominant event was gain in Xiang pig and Nuogu pig breeds,but it was not diverse in other three pig breeds.These results indicated that three CNV regions emerged with polymorphism in five pig breeds,which might have effects on gene expression in CNV regions and physiological function by dosage effect especially in Xiang pig,Nuogu pig and Kele pig breeds. |
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| Keywords: | copy number variation pig breeds Real-time quantitative PCR polymorphism |
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