| 子宫蓄脓对犬子宫内膜组织中IGF-2及IGF-1R蛋白表达的影响 |
| |
| 引用本文： | 杜淼,岳英伟,周朋洋,李爽,马文芝.子宫蓄脓对犬子宫内膜组织中IGF-2及IGF-1R蛋白表达的影响[J].中国畜牧兽医,2016,43(5):1263-1269. |
| |
| 作者姓名： | 杜淼 岳英伟 周朋洋 李爽 马文芝 |
| |
| 作者单位： | 1. 天津农学院动物科学与动物医学学院, 天津 300384;2. 国药集团(天津)医疗器械有限公司, 天津 300022 |
| |
| 基金项目： | 天津市财政专项项目《转基因家畜新品种培育、健康养殖新技术研究与开发利用》(2011CZZX) |
| |
| 摘 要： | 探讨IGF-2及IGF-1R蛋白质在子宫蓄脓的表达及与子宫蓄脓发病的相关性。采用免疫组织化学PV法与Western blotting检测5例正常子宫内膜(对照组)、19例子宫蓄脓(试验组)子宫内膜组织中IGF-2及IGF-1R的表达情况。结果表明,免疫组化与Western blotting检测结果一致,IGF-2和IGF-1R在蓄脓子宫组中的表达量极显著高于正常子宫(P<0.01)。对子宫蓄脓组中IGF-1R与IGF-2进行相关性分析,免疫组化结果表明IGF-1R与IGF-2之间低度相关(ｒ=0.423),Western blotting结果显示IGF-1R与IGF-2之间高度相关(ｒ=0.927)。结果提示,IGF-2阳性表达上调及IGF-1R的异常高表达,在子宫蓄脓的发生和发展过程中可能具有重要作用。
|
| 关 键 词： | IGF-2 IGF-1R 子宫蓄脓 免疫组化 Western blotting |
| 收稿时间： | 2015-10-21 |
Effect of Canine Pyometra on IGF-2, IGF-1R Proteins Expressions of Endometria |
| |
| DU Miao,YUE Ying-wei,ZHOU Peng-yang,LI Shuang,MA Wen-zhi.Effect of Canine Pyometra on IGF-2, IGF-1R Proteins Expressions of Endometria[J].China Animal Husbandry & Veterinary Medicine,2016,43(5):1263-1269. |
| |
| Authors: | DU Miao YUE Ying-wei ZHOU Peng-yang LI Shuang MA Wen-zhi |
| |
| Institution: | 1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China;2. SinoPharm (Tianjin) Medical EquiPment Co., Ltd., Tianjin 300022, China |
| |
| Abstract: | To investigate the expression of IGF-2 and IGF-1R protein in endometria of canine pyometra and analyze the possible correlation with the incidence of canine pyometra.Immunohistochemistry PV method and Western blotting were used to measure the IGF-2 and IGF-1R expression characteristic of 5 normal endometria and 19 endometria of canine pyometra.The results showed that the IGF-2 level of endometria from pyometra group was extremely significant higher than that of control group by immunohistochemistry and Western blotting (P<0.01).The positive expression rate of IGF-1R in endometria of canine pyometra compared with that in the normal endometria was extremely significant higher by immunohistochemistry and Western blotting (P<0.01).The correlation analysis of IGF-2 and IGF-1R in canine pyometra showed that the result of immunohistochemistry was low correlation (ｒ=0.423), the result of Western blotting was high correlation (ｒ=0.927).The data suggested that increased expression of IGF-2 and IGF-1R played an important role in occurrence of canine pyometra. |
| |
| Keywords: | IGF-2 IGF-1R canine pyometra immunohistochemistry Western blotting |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国畜牧兽医》浏览原始摘要信息 |
| 点击此处可从《中国畜牧兽医》下载免费的PDF全文 |
|
