Knockdown of survivin using siRNA promotes prostatic apoptosis in cancer cell DU145 |
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| Authors: | LIU Yan-bo SHEN Wei-gao LU Li-li GE He GAI Xiao-dong ZHAO Xue-jian |
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| Institution: | 1. Department of Pathophysiology, School of Basic Medical Sciences, Beihua University, Jilin 132013, China;2. The Prevention and Cure Center of Prostate Disease, Jilin University, Changchun 130021, China. E-mail: gaixiaodong@yahoo.com.cn; pro_2@jlu.edu.cn |
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| Abstract: | AIM: To investigate the promoting effects of survivin-siRNA on apoptosis of DU145 cells. METHODS: The DNA template coding siRNA against survivin was synthesized and recombinant plasmid pSi-sur was constructed. The recombinant and the two controls, liposome and pSi-scrambled plasmid, were transfected into DU145 cells. The expression of survivin in mRNA and protein was detected by RT-PCR and Western blotting, respectively. Apoptosis was measured by acridine orange staining, Annexin V-FITC labeled flow cytometry and TUNEL assay.RESULTS: Compared to the liposome control, the levels of survivin mRNA and protein in siRNA group were 0.28±0.07 and 0.34±0.05 (n=3, P<0.05) respectively 72 h after transfection. The apoptotic rate of the cells in pSi-sur group was significantly higher than that in two control groups as showed in acridine orange staining, Annexin V-FITC labeled flow cytometry and TUNEL assay.CONCLUSION: Survivin siRNA significantly inhibits the expression of survivin both in mRNA and protein levels, and induces apoptosis of DU145 cells in vitro. |
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