Diagnostic validity of cervical precancerous lesions by liquid-based cytology technique combined with high-risk HPV-DNA detection |
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| Authors: | WANG Ze-man CHEN Yu ZHENG Jian-peng |
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| Institution: | 1. Department of Gynecological Examination,The Second People's Hospital of Zhuhai City, Zhuhai 519020, China;
2. Department of Prevention and Health Care,The Second People's Hospital of Zhuhai City, Zhuhai 519020, China;
3. Department of Laboratory Medicine, The Second People's Hospital of Zhuhai City, Zhuhai 519020, China |
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| Abstract: | AIM: To investigate liquid-based cytology technique (LCT) combined with high-risk human papilloma virus DNA (HPV-DNA) detection for diagnosis of cervical precancerous lesions. METHODS: Screening of cervical cancer was performed by LCT combined with HPV-DNA detection in 6 521 women at the age of 19~65 in our hospital for physical examination from December 2007 to December 2010. Eighteen high-risk HPV isoform genes were detected by HPV typing gene chip detection system. The women with positive detection underwent colposcopic cervical biopsies. The women with negative detection also underwent colposcopic cervical biopsies if the operation was of their own accord. RESULTS: The LCT positive results (≥ASCUS) were observed in 152 cases and HPV positive results were obtained in 86 cases. The women with both 2 positive results were found in 42 cases. The women with both LCT and HPV negative results were determined in 6 325 cases. In 152 cases of LCT positive and 86 cases of HPV positive women, the positive results of pathological biopsy (≥CINI) were 112 and 68 cases, respectively. Thirty-four cases in the 42 cases of both LCT and HPV positive women were conformed by pathological biopsy. In 6 325 women with negative results of LCT and HPV detection, 2 000 were perform pathological biopsy according to their own accord and only 1 had positive result. The diagnostic sensitivity of LCT for cervical precancerous lesions was 76.19% with the specificity of 98.05%. The diagnostic sensitivity of HPV detection for precancerous lesions of uterine cervix was 46.26% with the specificity of 99.12%. Combination of the 2 methods to diagnose cervical precancerous lesions made the sensitivity increase to 99.32% with the specificity of 99.61%. CONCLUSION: Liquid-based cytology technique combined with high-risk HPV-DNA detection is better than each of the single technique for screening precancerous lesion of cervical carcinoma. |
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| Keywords: | Liquid-based cytology technique HPV-DNA detection Precancerous lesions of uterine cervix Validity |
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