Aged bone marrow stem cells can be rejuvenated by cell fusion with young bone marrow stem cells |
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| Authors: | LI Jiao CHEN Min-sheng YANG Wei-jian ZHANG Zhen-hui ZHONG Yun LIU Shi-ming |
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| Institution: | Department of Cardiology, The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou Institute of Cardiovascular Disease, Guangzhou 510260, China. E-mail: gzliushiming@126.com |
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| Abstract: | AIM: To investigate the function of aged bone marrow mesenchymal stem cells (BMSCs) fused with young BMSCs in mice. METHODS: The cell fusion model, which was made by C57BL/6 mouse BMSCs labeled with PKH26 membrane red fluorescence (young cells, age of 2-3 months, Y) and (old cells, age of 18-24 months, O), and young and old BMSCs of green fluorescent protein (GFP) transgenic C57BL/6 mouse, was established by the induction of polyethylene glycol 1500 (PEG 1500). The cell fusion rate and cell surface markers were detected by flow cytometry. The morphology and nuclear characteristics of the fused cells were observed under fluorescence microscopy. In this study, the age dependent changes in BMSCs proliferation and differentiation potential in Y group, O group, and another three fusion groups (Y-Y group, Y-O group, O-O group) were examined. The proliferation potentials in 5 groups were compared by counting cell numbers at days 2, 4, 6, and 8. The osteogenic and adipogenic differentiation potentials of the cells in 5 groups were determined by using standard differentiation procedures. RESULTS: The fusion rate of 30.45%±4.13% was obtained by PEG 1500 induction. No significant difference of the fusion rates in Y-Y, Y-O and O-O groups was observed. Fused BMSCs coincided with the common BMSCs were reactive to the BMSCs lineage-specific CD44, Sca-1 surface markers and negative for the hematopoietic stem cells (HSCs) lineage-specific surface markers such as CD34, CD117, CD31, and CD45. The percentage of increasing cell numbers in Y-O group was significantly higher than that in O-O group at days 2, 4, 6, and 8. The positive rate of the area stained with Alizarin red, which represents osteogenic differentiation potential of BMSCs, was significantly higher in Y-O group than that in O-O group ［(25.46%±1.52%) vs (13.85%±1.69%), P<0.01］. In Y-O group, the higher rate of the positive area stained with oil red O, which represents adipogenic differentiation potential of BMSCs, was observed as compared to that in O-O group ［(12.99%±2.61%) vs (6.03%±1.71%), P<0.05］. CONCLUSION: Aged bone marrow stem cells can be rejuvenated by cell fusion with young bone marrow stem cells, particularly the proliferation and differentiation potentials. |
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