Effect of oncogenic microRNA-106a on growth of normal gastric mucous epithelial cells and gastric cancer cells |
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| Authors: | LIAO Zi-wei DENG Hong-xia ZHANG Guo-ping ZHOU Hui GUO Jun-ming |
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| Institution: | Department of Biochemistry, Ningbo University School of Medicine, Ningbo 315211, China |
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| Abstract: | AIM: To investigate the oncogenic effect of microRNA-106a (miR-106a) on normal gastric mucous epithelial cells and gastric cancer cells.METHODS: The miR-106a mimic was transfected into normal gastric mucous epithelial cell line GES-1 using liposome. The change of cell growth was measured by MTT assay. The miR-106a inhibitor was transfected into gastric cancer cell lines MGC-803 and SGC-7901 using liposome, and the changes of cell cycle distribution and cell cycle-related protein expression were measured by flow cytometry and Western blotting,respectively. The growth of gastric cancer was also observed using nude mouse xenograft model. RESULTS: The miR-106a mimic increased the growth of GES-1 cells in a dose-dependent manner. By decreasing the expression of cyclin-dependent kinase (CDK) 1 and CDK2, the miR-106a inhibitor arrested MGC-803 cells at G0/G1 and G2/M phases. The miR-106a inhibitor also arrested SGC-7901 cells at G2/M phase by decreasing the expression of CDK1. The results of animal experiments showed that the miR-106a inhibitor significantly suppressed the tumor growth in a dose-dependent manner.CONCLUSION: miR-106a may play an important role in the development of gastric cancer. |
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| Keywords: | Stomach neoplasms microRNA-106a microRNA mimic microRNA inhibitor Cell cycle |
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