Effect of selenium compounds on ROS and NO production and NOS activity in human umbilical cord mesenchymal stem cells |
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| Authors: | ZOU Ying LING Qin-jie LIU Qiu-ying ZHANG Yi-bo GAO DONG-fang LU Qiao-ying LIU Jie ZHENG Wen-jie HUANG Zhi |
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| Institution: | 1. Department of Biological Engineering, College of Life Science & Technology, Jinan University, Guangzhou 510632, China;
2. Department of Chemistry, College of Life Science & Technology, Jinan University, Guangzhou 510632, China;
3. Biomedicine Research & Development Center, College of Life Science & Technology, Jinan University, Guangzhou 510632, China |
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| Abstract: | AIM: To investigate the effects of selenium (Se) compounds on the generation of nitric oxide (NO) and reactive oxygen species (ROS), and the activity of nitric oxide synthase (NOS) in umbilical cord mesenchymal stem cells (MSCs). METHODS: MSCs were cultured in the medium of DMEM/F12 containing 10% FBS and exposed to the compounds of selenium , selenocysteine (Se-Cys) or nano-selenium (Nano-Se) at concentrations of 0.1 μmol/L to 0.5 μmol/L. The concentration of NO and the activity of NOS in treated MSCs were detected by the method of nitrate reductase assay. The fluorescent intensity of ROS was determined by DCFH-DA probe. RESULTS: The production of NO was (18.13±6.80) μmol/L in the control MSCs,(20.93±5.68) μmol/L in the MSCs treated with Se(Ⅳ) at concentration of 0.1 μmol/L and (16.73±5.03) μmol/L in the MSCs treated with Se(Ⅳ) at concentration of 0.5 μmol/L for 24 h. The production of NO was (17.20±9.11) μmol/L (P<0.05) in the MSCs treated with Se(IV) at concentration of 0.1 μmol/L and (9.98±4.35) μmol/L (P<0.01) in the MSCs treated with Se(IV) at concentration of 0.5 μmol/L for 48 h, which was significantly lower than that in the control MSCs . No inhibitory effect of Nano-Se and Se-Cys on the production of NO in MSCs was observed. Compared with the control MSCs, Se(Ⅳ) at concentrations of 0.1 μmol/L and 0.5 μmol/L significantly inhibited the generation of ROS and the activity of NOS in MSCs at 24 h and 48 h. CONCLUSION: Se(Ⅳ) inhibits NO/ROS production and NOS activity in a dose-dependent manner in MSCs. |
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| Keywords: | Mesenchymal stem cells Selenium compounds Nitric oxide Nitric-oxide synthase Reactive oxygen species |
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