Effect of angiotensin-(1-7) on angiotensin II-induced rat renal interstitial fibroblast activation |
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| Authors: | ZHANG Xiao-cui HE Hong-yan XIA Ji-yi FAN Jun-ming OU San-tao LIU Jian |
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| Institution: | 1. Department of Nephropathy,The Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China;
2. Department of Pathology,The Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China;
3. Department of Molecular Immunity, The Affiliated Hospital of Luzhou Medical College, Luzhou 646000, China |
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| Abstract: | AIM: To explore the influence of angiotensin-(1-7) on angiotension II (Ang II)-induced activation and extracellular matrix secretion in rat renal interstitial fibroblasts (NRK-49F cells). METHODS: The NRK-49F cells were maintained and sub-cultured, then the cells were divided into control group, Ang II group, Ang-(1-7) group and Ang II+Ang-(1-7) group. The expression of α-smooth muscle actin(α-SMA),transforming growth factor β1 (TGF-β1) and insulin-like growth factor I(IGF-I) was detected by the method of immunocytochemistry when the cells were cultured for 72 h. The content of TGF-β1, IGF-I and collagen type I(Col I) in the cultured supernatants were measured by ELISA. RESULTS: In control group and Ang-(1-7) group, only basic expression of α-SMA and almost no expression of TGF-β1, IGF-I and Col I were observed. Compared with control group, the expression of α-SMA, TGF-β1, IGF-I and Col I was increased in Ang II group. Compared with Ang II group, the expression of α-SMA, TGF-β1, IGF-I and Col I was significantly decreased in Ang II+Ang-(1-7) group.CONCLUSION: Ang-(1-7) inhibits the activation of renal interstitial fibroblasts and decreases the Ang II induced secretion of Col I by suppressing TGF-β1 and IGF-I expression. |
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| Keywords: | Angiotensin-(1-7) Angiotensin II Renal interstitial fibroblast activation |
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