Polysaccharides of Dicliptera chinensis (L.) Nees. alleviates liver injury of GalN/LPS-induced fulminant hepatitis in rats |
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| Authors: | ZHANG Xiao-ling XIAO Sheng-jun RONG Ming-zhi SHEN Jie ZHANG Ke-feng GAO Ya ZHU Hua |
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| Institution: | 1.Department of Medical Function, 2Department of Pathophysiology, 3Department of Pathology, 4Laboratory of Pharmacology, Guilin Medical College, Guilin 541001, China;5.Department of Authentication of Chinese Medicine, Guangxi Traditional Chinese Medical University, Nanning 530001, China. E-mail: kent@glmc.edu.cn |
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| Abstract: | AIM: To investigate the liver protective effects of polysaccharides of Dicliptera chinensis (L.) Nees. on fulminant hepatitis caused by D-galactosamine (D-GalN)/lipopolysaccharide (LPS) in rats. METHODS: Male Wistar rats were divided into 4 groups (12 rats in each group), including normal control group, GalN/LPS-treated group, and two Dicliptera chinensis (L.) Nees. polysaccharides-treated groups (150 mg/kg, 300 mg/kg, ig, respectively). The Dicliptera chinensis (L.) Nees. polysaccharides and controlled normal saline (NS) were administered once 1 d for 6 d. One hour after the latest administration, all animals except for the animals in control group were administered with D-GalN (500 mg/kg, ip), then treated with LPS 1 h later. The mortality was observed at 6 h and 12 h after modeling. The serum samples were collected at 6 h and 12 h in the survival rats by retro-orbital sampling. All samples were measured for ALT, AST, TNF-α and IL-1β. The samples collected at 12 h were also detected for NO. At 12 h, all survival rats were sacrificed and liver section was prepared for histological evaluation. RESULTS: Pretreatment with polysaccharides of Dicliptera chinensis (L.) Nees. significantly improved the histopathological changes and attenuated GalN/LPS-induced severe hepatotoxicity as evidenced by decrease in the levels of ALT and AST. The polysaccharides of Dicliptera chinensis (L.) Nees. inhibited the elevated levels of TNF-α, IL-1β and NO. CONCLUSION: Using Dicliptera chinensis (L.) Nees. polysaccharides as anti-inflammatory reagent provides a definite protective way against fulminant hepatitis caused by GalN/LPS in rat. |
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