Inhibitory effect of cholecystokinin-octapeptide on production of hydrogen sulfide in lung of LPS-induced lung injury rats |
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| Authors: | HUANG Xin-li ZHOU Xiao-hong TIAN Feng-jun XIAN Xiao-hui LING Yi-ling |
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| Institution: | Department of Pathophysiology, Hebei Medical University, Shijiazhuang 050017, China. E-mail:zzxxhh2007@126.com |
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| Abstract: | AIM: To investigate the role of hydrogen sulfide (H2S) in the cholecystokinin octapeptide (CCK-8) attenuating lipopolysaccharide (LPS)-induced lung injury. METHODS: A rat model of lung injury induced by intravenous injection of LPS was developed. Male Wistar rats were divided into normal control group, LPS group, LPS+CCK-8 group and CCK-8 group. Six hours after LPS injection, partial pressure of oxygen in the arterial blood (PaO2), H2S content and cystathionine-γ-lyase (CSE) activity in lung tissue were detected. The mRNA expression of CSE in lung tissue was determined by RT-PCR; the structure of lung tissues was observed under optical microscope. RESULTS: Compared to normal control rats, the LPS-treated rats had significantly decreased PaO2 level, increased index of quantitative assessment (IQA) score, while H2S content, CSE activity and the mRNA expression of CSE in lung tissue were significantly increased (all P<0.05). Administration of CCK-8 into LPS-treated rats increased the PaO2 level and alleviated the degree of lung injury (measured by IQA score). In addition, CCK-8 decreased H2S content, CSE activity, and the mRNA expression of CSE (all P<0.05). No significant difference of the above-mentioned parameters between CCK-8 group and normal control group was observed. CONCLUSION: CCK-8 reduces LPS-induced lung injury through inhibiting the generation of endogenous H2S. |
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