| 96孔板氯霉素分子印迹膜的制备 |
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| 引用本文： | 杜幸洁,张峰,张慧晓,文永佳,萨仁托雅.96孔板氯霉素分子印迹膜的制备[J].大连海洋大学学报,2014(1):57-60. |
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| 作者姓名： | 杜幸洁 张峰 张慧晓 文永佳 萨仁托雅 |
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| 作者单位： | 大连海洋大学农业部北方海水增养殖重点实验室,辽宁大连116023 |
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| 基金项目： | 辽宁省农业公攻关计划项目（2011215004） |
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| 摘 要： | 对96孔板上氯霉素分子印迹膜的制备及其吸附性能进行了研究,并通过试验优化了该分子印迹膜的制备方法.以氯霉素为模板分子,四氢呋喃为致孔剂,偶氮二异丁腈为引发剂,采用紫外灯引发聚合在MaxiSorp 96孔板上合成氯霉素分子印迹聚合膜.结果表明,当模板分子、功能单体（甲基丙烯酸二乙基氨基乙酯）、交联剂（乙二醇二甲基丙烯酸酯）的摩尔比为1∶4∶1,模板分子物质的量为0.25 mmol,溶剂的量为5 mL时,合成的氯霉素分子印迹膜对氯霉素的吸附效果最佳,可在50 min内达到吸附平衡,当氯霉素的初始浓度为120 mg/L时,吸附量可达1.51μg/孔.吸附特异性试验结果表明,当氯霉素、甲砜霉素和氟甲砜霉素的初始浓度为50 mg/L时,该膜对氯霉素的吸附量为0.83μg/孔,而对甲砜霉素和氟甲砜霉素的吸附量分别仅为0.49、0.40μg/孔.研究表明,将该氯霉素分子印迹膜作为人工抗体代替氯霉素的生物抗体,采用直接竞争原理,应用化学发光免疫法检测氯霉素,具有可行性.
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| 关 键 词： | 氯霉素 分子印迹聚合膜 人工抗体 化学发光免疫法 |
Preparation of chloramphenicol molecularly imprinted film on 96-well plates |
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| Institution: | DU Xing-jie, ZHANG Feng, ZHANG Hui-xiao, WEN Yong-jia, SAtlEN Tuo-ya (Key Laboratory of Marieulture ＆ Stock Enhancement in North China＇s Sea, Ministry of Agriculture, Dalian Ocean University, Dalian 116023, China) |
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| Abstract: | A chloramphenicol（CAP） molecularly imprinted film was prepared on the surface of MaxiSorp polystyrene 96-well plates using chloramphenicol as molecular template,tetrahydrofuran as solvent and azobisisobutyronitrile as initiator with a UV lamp,and the preparation conditions were optimized by a series of experiments.The results showed that the imprinted film exhibited good adsorption at ratio of the template,functional monomer （EDAEM） and cross-linker（EGDMA） =1∶4∶1,the template dosage of 0.25 mmol and the solvent of 5 mL.The adsorption equilibrium was found within 50 min and the adsorption of 1.51 μg/well was observed at initial chloramphenicol concentration of 120 mg/L.The chloramphenicol adsorption of 0.83 μg/well on the imprinted film was observed at the initial CAP,TAP and FF concentration of 50 mg/L.The florfenicol adsorption of only 0.40/well,and thiamphenicol adsorption of 0.49 μg/well were found at that CAP,TAP and FF concentration,indicating that the imprinted film can be used as an artificial antibody in chemiluminescence immunoassay based on the principle of direct competition for detection of chloramphenicol. |
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| Keywords: | chloramphenicol molecularly imprinted film artificial antibody chemiluminescence immunoassay |
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