| 大白菜DH群体TuMV抗性的QTL定位与分析 |
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| 引用本文： | 张晓伟,原玉香,王晓武,孙日飞,武剑,谢从华,蒋武生,姚秋菊.大白菜DH群体TuMV抗性的QTL定位与分析[J].园艺学报,2009,36(5):731-736. |
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| 作者姓名： | 张晓伟 原玉香 王晓武 孙日飞 武剑 谢从华 蒋武生 姚秋菊 |
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| 作者单位： | (华中农业大学园艺林学学院, 武汉430070; 河南省农业科学院园艺研究所, 郑州450002; 中国农业科学院蔬菜花卉研究所, 北京100081) |
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| 基金项目： | 国家高技术研究发展计划(863计划)，'国家科技支撑计划子课题，河南省重大科技攻关项目 |
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| 摘 要： | 利用已构建的包含287个标记位点的遗传图谱的大白菜DH群体，采用多模型QTL作图的方法，通过苗期人工接种TuMV-C4株系对来自抗病亲本Y195 93和感病亲本Y177-12的DH群体的TuMV抗性进行QTL分析。共检测到3个QTLs，分别位于R03、R04和R06连锁群上，解释的表型变异在10.5%~21.9%之间，3个QTLs可解释40.0％的表型总变异。其中位于R04上命名为Tu-2的QTL解释的表型变异最高，为21.9%，其余两个位于R03和R06上的QTLs，分别解释10.5%和14.5%的表型变异。
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| 关 键 词： | 大白菜 TuMV QTL定位 DH群体 |
| 收稿时间： | 2008-10-25 |
QTL Mapping for TuMV Resistance in Chinese Cabbage [Brassica campestris L.ssp.pekinensis(Lour.)Oissom] |
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| ZHANG Xiao-wei,YUAN Yu-xiang,WANG Xiao-WU,SUN Ri-fei,WU Jian,XIE Cong-hua,JIANG Wu-sheng,YAO Qiu-ju.QTL Mapping for TuMV Resistance in Chinese Cabbage [Brassica campestris L.ssp.pekinensis(Lour.)Oissom][J].Acta Horticulturae Sinica,2009,36(5):731-736. |
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| Authors: | ZHANG Xiao-wei YUAN Yu-xiang WANG Xiao-WU SUN Ri-fei WU Jian XIE Cong-hua JIANG Wu-sheng YAO Qiu-ju |
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| Institution: | (College of Horticulture and Forestry, Huazhong Agricultural University, Wuhan 430070, China; Institute of Horticulture,Henan Academ y of Agricultural Sciences, Zhengzhou 450002, China; Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing 100081, China ) |
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| Abstract: | One hundred and eighty-three doubled hap loid (DH) lines derived from the cross of two Chinese cabbage Brassica campestris L. ssp. pekinensis (Lour. ) Olssom ] DH lines, Y177212 (TuMV-susceptible) and Y195293 ( TuMV-resistant) , were used to map quantitative trait loci (QTL) related to TuMV resistance. TuMV resistance was evaluated for young seedlings by inoculating true leaves with TuMV-C4 strain.Based on the reported genetic map with 287 loci, 3 QTLs were detected for TuMV resistance, localizing on linkage group s R03, R04 and R06, respectively. The most significant of the identified QTL locates on R04,named Tu-2, exp laining 21.9% of the phenotyp ic variance. The other two QTL s mapped on R03 and R06, explaining 10.5% and 14.5% of the phenotyp ic variance, respectively. The three QTL s together exp lained40.0% of the TuMV resistance variance. |
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| Keywords: | TuMV |
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