|
|||||
|
|
| 茶花粉酶法破壁工艺提高提取物抗氧化活性及多酚含量 | |
| 引用本文： | 董亚婷,杨远帆,倪 辉,彭文君.茶花粉酶法破壁工艺提高提取物抗氧化活性及多酚含量[J].农业工程学报,2013,29(21):288-294. |
| 作者姓名： | 董亚婷 杨远帆 倪 辉 彭文君 |
| 作者单位： | 1. 集美大学生物工程学院,厦门,361021 2. 集美大学生物工程学院，厦门 361021; 厦门市食品生物工程研究中心，厦门 361021 3. 中国农业科学院蜜蜂研究所,北京,100093 |
| 基金项目： | 国家蜂产业技术体系科研基金项目（30771636）；集美大学科研创新团队基金（No. 2010A006）。 |
| 摘 要： | 为了建立蜂花粉抗氧化活性物质的提取工艺并探索其抗氧化活性与多酚含量的关系，该文研究了破壁用酶、提取溶剂、超声波处理对茶花粉提取液多酚含量及其抗氧化活性的影响。结果表明，茶花粉经纤维素酶破壁后其上清液具有较高的还原力和DPPH自由基清除能力；与温水和酸处理相比，纤维素酶破壁沉淀物经乙醇提取后，溶液多酚含量较高、抗氧化活性较强；茶花粉不同提取方式多酚含量与2种抗氧化活性指标具有一定的相关性（r=0.8685及r=0.7600）（p＞0.05）；与乙醇提取和水提取相比，纤维素酶破壁处理结合乙醇提取将茶花粉的多酚含量、还原力及DPPH自由基清除能力分别提高1.82倍、2.17倍、1.4倍和1.56倍、1.38倍、11倍，且二者对混合物还原力、DPPH自由基清除能力及多酚含量的贡献比例分别为1.6：1、3：1、1.08：1。该研究结果可为花粉资源的开发利用提供参考。 |
| 关 键 词： | 提取 纤维素酶 乙醇 茶花粉 破壁 多酚 抗氧化 |
| 收稿时间： | 2013/5/20 0:00:00 |
| 修稿时间： | 2013/9/28 0:00:00 |
Enzymatic cell wall disruption process improves antioxidant activity and polyphenol content of camellia pollen extracts |
|
| Dong Yating,Yang Yuanfan,Ni Hui and Peng Wenjun.Enzymatic cell wall disruption process improves antioxidant activity and polyphenol content of camellia pollen extracts[J].Transactions of the Chinese Society of Agricultural Engineering,2013,29(21):288-294. | |
| Authors: | Dong Yating Yang Yuanfan Ni Hui and Peng Wenjun |
| Institution: | 1. School of Bioengineering, Jimei University, Xiamen 361021, China3. Food Bio-engineering Research Center of Xiamen, Xiamen 361021, China;1. School of Bioengineering, Jimei University, Xiamen 361021, China3. Food Bio-engineering Research Center of Xiamen, Xiamen 361021, China;2. Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China |
| Abstract: | Abstract: Bee pollen is used as a dietary supplement which contains nearly all nutrients required by humans. It has been used as a folk medicine for centuries to alleviate or cure conditions such as colds, flu, ulcers, premature ageing etc. Related researches have indicated that pollen has stronger antioxidant activity home and abroad. It has been pointed out that polyphenols have unique effect on human health and are the most widespread biodiversity natural material, which is widely recognized in the scientific community. Bee pollen is rich in polyphenol compounds, which can be used as potential antioxidant. Because of the solid pollen wall, some biological active ingredients and nutrients are not effectively absorbed by human body, thus the cell wall disruption is essential. Although research has manifested that the enzymatic cell wall disruption can increase the extracts antioxidant, few studies have conducted on the basic active ingredients and the extraction progress. In order to establish the extraction progress of pollen antioxidants and explore the relative correlation between the polyphenol contents and its antioxidant activity, a series experiments were conducted on the influence of antioxidant ability and the polyphenol contents with different factors which were cell wall disruption enzyme, extraction solvent, and ultrasonication. The results showed that compared with the alkalase, the supernates from celluase progress showed higher free-radical scavenging ability of DPPH and reducing power; compared with warm water and pH3.7 citric acid-phosphate buffer, ethanol extracts from the sediments of the celluase showed higher polyphenol contents and free-radical scavenging ability. It showed some correlation coefficient between total phenols and two antioxidant indices in camellia pollen was 0.8685 and 0.7600 respectively (p>0.05). In terms of ethanol and water extract, cell wall disruption with celluase and ethanol extract increased the polyphenol contents reducing power and the scavenging ability of DPPH by 1.82, 2.17, 1.4 times and 1.56, 1.38, 11 times respectively. The contribution to the reducing power, DPPH radical scavenging ability and the polyphenol content of the mixture were 1.6:1, 3:1, 1.08:1. The results provide some reference to the development and the utilization of the pollen resource. |
| Keywords: | extraction cellulose alcohols camellia pollen cell wall disruption polyphenols antioxidant |
| 本文献已被 万方数据 等数据库收录！ | |
| 点击此处可从《农业工程学报》浏览原始摘要信息 | |
| 点击此处可从《农业工程学报》下载免费的PDF全文 | |