| 高效液相色谱法检测牛乳中的磺胺类药残留量 |
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| 引用本文： | 孔庆德.高效液相色谱法检测牛乳中的磺胺类药残留量[J].畜牧兽医杂志,2013,32(3):4-6. |
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| 作者姓名： | 孔庆德 |
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| 作者单位： | 武威市农产品质量安全监督管理站,甘肃武威,733000 |
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| 摘 要： | 为建立HPLC法测定牛奶中磺胺类药残留量的方法。样品经乙腈处理沉淀蛋白质后取上清液浓缩,以乙腈-水(体积比为1∶1)为流动相进行高效液相色谱分析。结果表明4种磺胺类药分离效果良好,平均回收率范围81.6%～95.8%,相对标准偏差为1.5%～4.2%,最低检出限达1.0～2.0ng/mL。可见该检测法具有灵敏、准确、精密、无杂质干扰等优点。
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| 关 键 词： | 牛奶 磺胺类药 残留量 高效液相色谱法 |
Milk Sulfonamides Residues Detection Using The Method of High Performance Liquid Chromatography |
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| KONG Qing-de.Milk Sulfonamides Residues Detection Using The Method of High Performance Liquid Chromatography[J].Journal of Animal Science and Veterinary Medicine,2013,32(3):4-6. |
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| Authors: | KONG Qing-de |
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| Institution: | KONG Qing-de(Wuwei City Agricultural Products Safety Administration and Supervision Station,Wuwei Gansu 73300,China) |
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| Abstract: | The objective of this study was to establish the method of evaluating sulfonamides residues in milk. Supernatant was collected from samples with protein extracted by acetonitrile,then high performance liquid chromatograph （HPLC） used to analyze with acetonitrile water （concentration, volume ratio of 1 ： 1） as mobile phase. The results indicated that good separation effects for 4 kinds of sulphonamides, average recovery rate was 81. 60/00 - 95. 8%, the relative standard deviation was 1.5 %-4.2%, the minimum detectable limit was 1 - 2.0 ng / mL. Therefore, the above method has several advantages inclu- ding sensitive,accurate, precise, no impurity interference. |
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| Keywords: | milk sulphonamides residue HPLC |
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