| 航天苜蓿中黄酮提取工艺及抑菌活性研究 |
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| 引用本文： | 廖天录,方梅,陈虹,赵强.航天苜蓿中黄酮提取工艺及抑菌活性研究[J].畜牧兽医杂志,2013,32(3):17-18,20. |
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| 作者姓名： | 廖天录 方梅 陈虹 赵强 |
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| 作者单位： | 1. 甘肃工业职业技术学院化工学院,甘肃天水,741025
2. 甘肃农业大学动物医学院 |
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| 摘 要： | 用索氏回流进行正交提取航天苜蓿中的黄酮,以510nm最大吸光度进行可见光谱检测,测定黄酮含量,并对两种供试菌进行体外抑菌活性测定。结果表明,通过单因素实验确定的各因素影响能力大小为:pH>浸泡时间>料液比,含量最高为10.04%;对大肠埃希氏杆菌和金黄色葡萄球菌的MIC为0.40mg/mL和0.70mg/mL。
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| 关 键 词： | 航天 苜蓿 黄酮 抑菌 |
Study on Extraction Technology and Antibacterial of Flavonoids from Space Breeding Alfalfa |
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| LIAO Tian-lu
,
FANG Mei
,
CHEN Hong
,
ZHAO Qiang.Study on Extraction Technology and Antibacterial of Flavonoids from Space Breeding Alfalfa[J].Journal of Animal Science and Veterinary Medicine,2013,32(3):17-18,20. |
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| Authors: | LIAO Tian-lu FANG Mei CHEN Hong ZHAO Qiang |
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| Institution: | 1. Department of Chemistry Engineering ,Gansu Industry Polytechnic College, Tianshui 741025, China 2. College of Veterinary Medicine, GanSu Agricultural University) |
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| Abstract: | To 510 nm biggest spectrophotometry visible spectrum for testing, methods with backflow quadrature, determine flavonoids content of space alfalfa and the two selected bacteria for in vitro antibacterial activity. Through the single factor to determine the influence of various factors, the results show pH 〉 Macerating time 〉 Liquid to solid ratio and the highest fla- vonoids content is 10.04% ; MIC on E. coli and S. aureus is 0.40 mg/mL and 0.70 mg/mL. |
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| Keywords: | space flight alfalfa flavonoids antibacterial |
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