| 玉米高赖氨酸含量基因型离体筛选方法与应用 |
| |
| 引用本文： | 杨靖,刘改凤,曹银萍,李友勇.玉米高赖氨酸含量基因型离体筛选方法与应用[J].玉米科学,2013,21(2):106-111. |
| |
| 作者姓名： | 杨靖 刘改凤 曹银萍 李友勇 |
| |
| 作者单位： | 河南科技学院/河南省高等学校作物分子育种重点学科开放实验室, 河南 新乡 453003;四川农业大学, 成都 611130;河南科技学院/河南省高等学校作物分子育种重点学科开放实验室, 河南 新乡 453003;河南科技学院/河南省高等学校作物分子育种重点学科开放实验室, 河南 新乡 453003;河南科技学院/河南省高等学校作物分子育种重点学科开放实验室, 河南 新乡 453003 |
| |
| 基金项目： | 河南省教育厅基础研究项目(2011B21000) |
| |
| 摘 要： | 设不同浓度赖氨酸(L)加苏氨酸(T)的培养基, 将opaqure-2等32个玉米自交系种子的胚接种在培养基上发芽, 同时测定种子中游离氨基酸和总氨基酸含量, 检测胚生长对L+T的抗性与种子中赖氨酸含量关系, 筛选鉴定玉米种子中高赖氨酸含量的有效方法。结果表明, opaqure-2对L+T有较强抗性, 子粒中有较高含量游离赖氨酸;普通玉米自交系抗L+T能力较弱, 种子中游离氨基酸含量较低;在旅大红骨类群中检测到对L+T有较强抗性的基因型, 子粒中有较高含量的赖氨酸和其他氨基酸。玉米中存在典型的天冬族氨基酸生物合成的反馈抑制代谢途径, 试管L+T胁迫方法可用于筛选和鉴定玉米高赖氨酸基因型。
|
| 关 键 词： | 玉米 赖氨酸 苏氨酸 离体筛选 |
| 收稿时间： | 2012/6/11 0:00:00 |
Method and Application for Selecting High Content Lysine Genotypes in vitro in Maizeq |
| |
| YANG Jing,LIU Gai-feng,CAO Yin-ping.Method and Application for Selecting High Content Lysine Genotypes in vitro in Maizeq[J].Journal of Maize Sciences,2013,21(2):106-111. |
| |
| Authors: | YANG Jing LIU Gai-feng CAO Yin-ping |
| |
| Institution: | Henan Institute of Science and Technology/Key Discipline Opening Laboratory of Crop Molecular Breeding of Henan Province University, Xinxiang 453003;Sichuan Agricultural University, Chengdu 611130, China;Henan Institute of Science and Technology/Key Discipline Opening Laboratory of Crop Molecular Breeding of Henan Province University, Xinxiang 453003;Henan Institute of Science and Technology/Key Discipline Opening Laboratory of Crop Molecular Breeding of Henan Province University, Xinxiang 453003;Henan Institute of Science and Technology/Key Discipline Opening Laboratory of Crop Molecular Breeding of Henan Province University, Xinxiang 453003 |
| |
| Abstract: | In order to explore the method of selection and identification high lysine content genotypes in maize, the method of comparing the relationship between the resistance to lysine(L) and threonine(T) in vitro and the content of lysine in the grains was carried in this paper. The embryos of 32 varieties of inbred lines of maize include opaqure-2 were planted on medium in which different concentration of lysine and threonine were added, and at the same time, the content of free lysine, threonine, and other essential amino acids in the grains of partial varieties were detected by HPLC. The results showed that high resistance to L+T in vitro and high content of free lysine in seeds exhibited in opaqure-2. But for the majority of inbred lines, both the resistance and the content were low. It was also found that some inbred line belonging to the group of Lvdahonggu had high lysine content and high resistance to lysine in vitro. These results suggested that there was a positive relationship between the resistance to lysine plus threonine in vitro and the content of lysine in the grains in maize, and typical feedback inhibition metabolic characteristics in the pathways of aspartic family amino acid biosynthesis. The stress of lysine plus threonine in vitro could be used for selecting a high resistant genotype which may contain higher content lysine and other essential amino acids in grains of maize. |
| |
| Keywords: | Maize Lysine Threonine Selection in vitro |
|
| 点击此处可从《玉米科学》浏览原始摘要信息 |
| 点击此处可从《玉米科学》下载免费的PDF全文 |
|
