| 二、四倍体不结球白菜子叶再生能力及其与内源激素的关系 |
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| 引用本文： | 孙敏红,张蜀宁.二、四倍体不结球白菜子叶再生能力及其与内源激素的关系[J].南京农业大学学报,2006,29(2):25-28. |
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| 作者姓名： | 孙敏红 张蜀宁 |
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| 作者单位： | 1. 南京农业大学蔬菜研究所,江苏,南京,210095;中南林业科技大学资源环境学院,湖南,长沙,410004
2. 南京农业大学蔬菜研究所,江苏,南京,210095 |
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| 摘 要： | 以6个同源四倍体和2个二倍体不结球白菜（Brassica campestris ssp．chinensis Makino）的子叶为外植体，正交设计筛选不定芽诱导优化培养基，测定二、四倍体不结球白菜子叶内源生长素IAA（吲哚-3-乙酸）和细胞分裂素ZR3（玉米素核苷类）的含量及ZRs／IAA比值大小，探讨再生能力与内源激素的相关性。结果表明：四倍体不定芽再生率变化范围是33．4％-83．3％，2个二倍体材料的最高再生率分别是33．3％和80．3％。四倍体白菜内源激素ZRs和IAA含量高于其相应二倍体白菜。用2种方法确定四倍体材料再生能力与内源激素含量的相关性，结果表明再生能力与ZRs／IAA比值显著相关，相关系数分别为0．9428和0．8857。
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| 关 键 词： | 不结球白菜 同源四倍体 二倍体 再生能力 内源激素 相关性 |
| 文章编号： | 1000-2030(2006)02-0025-04 |
| 收稿时间： | 06 30 2005 12:00AM |
| 修稿时间： | 2005年6月30日 |
The regeneration capacity of cotyledons of autotetraploid and diploid non-heading Chinese cabbage and its relation to the endogenous hormones |
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| SUN Min-hong,ZHANG Shu-ning.The regeneration capacity of cotyledons of autotetraploid and diploid non-heading Chinese cabbage and its relation to the endogenous hormones[J].Journal of Nanjing Agricultural University,2006,29(2):25-28. |
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| Authors: | SUN Min-hong ZHANG Shu-ning |
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| Institution: | 1. Institute of Vegetable, Nanjing Agricultural University, Nanjing 210095, China; 2. College of Resource and Environment, Central South Forestry University, Changsha 410004, China |
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| Abstract: | The cotyledons of six autotetraploid and two diploid non-heading Chinese cabbage used as explants,the optional medium of adventitious shoot regeneration was made by orthogonal design,and the regeneration capacity of explants of all materials was arranged.The content of endogenous hormones(ZR_S and IAA)and ZR_S/IAA were tested,studied the relativity between regeneration capacity and endogenous hormones.The experimental results showed that the regeneration capacity of adventitious shoot of autotetraploid non-heading Chinese cabbage was ranged from 33.4%83.3%,while the highest regeneration frequency of two diploid was 33.3%and 80.3% respectively.The content of endogenous hormones of autotetraploid was higher than that of their diploid.The relativity between the content of endogenous hormones and regeneration capacity was studied using two methods.And the results showed that there was significant relativity between regeneration capacity and the ratio of ZR_S/IAA,and the relativity coefficient were 0.948 2 and 0.885 7 respectively. |
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| Keywords: | Brassica campestris ssp chinensis Makino autotetraploid diploid regeneration capacity endogenous hormones relativity |
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