| 植物源抗病毒制剂 VA 对烟草花叶病毒(TMV)体外钝化作用的初步研究 |
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| 引用本文： | 李红霞,康绍兰,李兴红.植物源抗病毒制剂 VA 对烟草花叶病毒(TMV)体外钝化作用的初步研究[J].河北农业大学学报,2000,23(4). |
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| 作者姓名： | 李红霞 康绍兰 李兴红 |
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| 作者单位： | 河北农业大学农学院!河北保定071001(李红霞，康绍兰)，北京市农林科学院!北京100000(李兴红)，河北省隆尧县农业局!河北隆尧055300(耿同茹) |
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| 基金项目： | 河北省自然科学基金资助! (399110 ) |
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| 摘 要： | 用植物源抗病毒制剂VA与烟草花叶病毒 (TMV)混合 ,经枯斑半叶法接种 ,结果证明 ,VA对TMV有较强的体外钝化作用 ,体外钝化后的枯斑抑制率为 80 .0 % ;用苯酚氯仿法提取TMV的核酸后 ,按一定的比例与VA混合 ,经枯斑半叶法接种测得枯斑抑制率为 43.8%～ 77.9% ;TMV与VA混合后用RNase处理 ,测得侵染率为 78.6 % ,比未用RNase处理的对照降低了 2 1.4% ,由此说明VA具有一定的体外脱衣壳作用 ;又经电镜观察发现VA可使部分TMV粒体发生断裂或粗细不均、略变弯曲等畸变现象。
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| 关 键 词： | 植物源抗病毒制剂VA 烟草花叶病毒(TMV) 体外钝化作用 |
A preliminary study on the inactivation in vitro to tobacco mosaic virus (TMV) by anti viral agent VA from plants |
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| LI Hong xia ,KANG Shao lan ,LI Xing hong ,GENG Tong ru.A preliminary study on the inactivation in vitro to tobacco mosaic virus (TMV) by anti viral agent VA from plants[J].Journal of Agricultural University of Hebei,2000,23(4). |
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| Authors: | LI Hong xia KANG Shao lan LI Xing hong GENG Tong ru |
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| Institution: | LI Hong xia 1,KANG Shao lan 1,LI Xing hong 2,GENG Tong ru 3 |
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| Abstract: | VA , a anti viral agent from plants, could inactivate TMV in vitro , when it was mixed with TMV and inoculated by means of half leaf. The inhibition rate of necrotic local lesions was 80.0%. Ribonucleic acid extracted from TMV was mixed with VA in proportion, then inoculated onto leaves of tobacco. The experimental results showed that the inhibition rates of necrotic local lesions were from 43.8% to 77.9%. While RNase was added to the mixture of TMV and VA, the infection rate of TMV was 78 6%, decreased by 21 4% in contrast with the check. Meanwhile, it was showed that VA broke or made TMV particles become non equipollent thickness or curve slightly through transmission electron microscope (TEM). |
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| Keywords: | anti viral agent VA from plants tobacco mosaic virus inactivation in vitro |
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