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| 不同培养体系对牛胚胎体外发育的影响 | |
| 引用本文： | 成文敏,戴蕴平,朱士恩,李荣,田见晖.不同培养体系对牛胚胎体外发育的影响[J].中国畜牧杂志,2005,41(10):9-11. |
| 作者姓名： | 成文敏 戴蕴平 朱士恩 李荣 田见晖 |
| 作者单位： | 1. 中国农业大学动物科技学院,北京,100094 2. 农业与生物技术国家重点实验室,北京,100094 3. 中国农业大学动物科技学院,北京,100094;农业与生物技术国家重点实验室,北京,100094;农业部畜禽遗传育种重点开放实验室,北京,100094 4. 北京嘉捷康宁生物工程技术责任有限公司,北京,100094 5. 中国农业大学动物科技学院,北京,100094;农业部畜禽遗传育种重点开放实验室,北京,100094 |
| 基金项目： | 北京市科委重点项目(H022020060420)，高校博士点基金项目(20020019031) |
| 摘 要： | 采用离子霉素和6-二甲氨基嘌呤(6-DMAP)对牛体外成熟卵母细胞进行联合激活,激活后采用不同的培养体系进行体外培养,观察不同的培养液对牛孤雌激活胚体外发育能力的影响。3种培养体系分别为:A(0~48 h:CR1aa+3 mg/mL BSA;48 h~7 d:CR1aa+10%FBS),B(连续7 d均为SOFaa+3 mg/mL BSA),C(0~5 d:SOFaa+3 mg/mL BSA;6~7 d:SOFaa+10%FBS)。结果表明:3种培养液对卵裂率没有显著性影响,分别为87.22%,94.33%和91.30%,但囊胚发育率存在显著性差异,以A效果最好,其囊胚发育率为25.56%;C次之,囊胚发育率为11.80%;B最低,囊胚发育率为3.55%。之后选择最佳的培养液进行体外受精实验,结果表明CR1aa可用做牛胚胎体外生产的培养液。 |
| 关 键 词： | 畜牧学 体外培养 孤雌激活 体外受精 卵母细胞 牛 |
| 文章编号： | 0258-7033（2005）10-0009-03 |
| 修稿时间： | 2004年2月18日 |
Effect of Different Culture Systems on in vitro Development of Bovine Embryos |
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| CHENG Wen-min,DAI Yun-ping,ZHU Shi-en,LI Rong,TIAN Jian-hui.Effect of Different Culture Systems on in vitro Development of Bovine Embryos[J].Chinese Journal of Animal Science,2005,41(10):9-11. | |
| Authors: | CHENG Wen-min DAI Yun-ping ZHU Shi-en LI Rong TIAN Jian-hui |
| Abstract: | In vitro matured oocytes were activated with ionomycin and 6-DMAP.After activating,this study was conducted to investigate the effect of three in vitro culture systems on the development of parthenogenetic bovine embryos and to observe the development of bovine oocytes fertilized in vitro in the optimal culture system.These culture systems were: A((0～48 h): CR1_(aa)+3 mg/mL BSA;48 h～7 d: CR1_(aa)+10% FBS),B((0～7 d): SOF_(aa)+3 mg/mL BSA),C(0～5 d: SOF_(aa)+3 mg/mL BSA;6～7 d: SOF_(aa)+10% FBS).The result showed that there were no significant differences(P>(0.05)) in the cleavage rates of embryos in culture systems A(87.22%),B(94.33%) and C((91.30%)),but the blastocyst rates in culture systems A(25.56%),B(3.55%) and C(11.80%) differed significantly(P<0.05),and that the optimal culture system(A) could be served as the culture medium of in vitro produced(IVP) bovine embryos. |
| Keywords: | animal science in vitro culture parthenogenesis in vitro fertilization oocytes bovine |
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