| 非洲紫罗兰的核型分析 |
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| 引用本文： | 孙永健,陈小强,孙宁,陈杰,张磊,张乃楠.非洲紫罗兰的核型分析[J].天津农学院学报,2010,17(4):5-8. |
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| 作者姓名： | 孙永健 陈小强 孙宁 陈杰 张磊 张乃楠 |
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| 作者单位： | [1]天津农学院农学系,天津300384 [2]天津天狮学院,天津301700 [3]长沙市口腔医院,长沙410005 |
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| 基金项目： | 天津市科技支撑计划重点项目 |
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| 摘 要： | 将低渗技术引入染色体压片法,首次对非洲紫罗兰(Saintpaulia ionantha)缟花品种"Sunray Trail"进行了核型分析,并摸索了适用于该试材的预处理方法,为与之有关的细胞学分类、分子育种、新品种培育及转基因的细胞学检测等方面提供了细胞学依据。结果表明,供试材料为4倍体品种,其核型公式为2n=4x=28=16 m+8 sm+4 T;核型类型属于2B型;核不对称系数为63.3%;最佳预处理方法为在4℃下用0.05%的秋水仙素处理根尖2 h。
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| 关 键 词： | 核型分析 非洲紫罗兰 染色体压片法 去壁低渗 秋水仙素 |
Karyotype Analysis of Saintpaulia ionantha |
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| SUN Yong-jian,CHEN Xiao-qiang,SUN Ning,CHEN Jie,ZHANG Lei,ZHANG Nai-nan.Karyotype Analysis of Saintpaulia ionantha[J].Journal of Tianjin Agricultural College,2010,17(4):5-8. |
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| Authors: | SUN Yong-jian CHEN Xiao-qiang SUN Ning CHEN Jie ZHANG Lei ZHANG Nai-nan |
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| Institution: | 1. Department of Agronomy, Tianjin Agricultural University, Tianjin 300384, China. 2. Tianjin Tianshi College, Tianjin 301700, China; 3. Changsha Dental Hospital, Changsha 410005, China) |
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| Abstract: | The hypotonic treatment was introduced into the normal chromosome press part in ＂Sunray Trail＂ of Saintpaulia ionantha for the first time, and the steps and operating time were adjusted. This study can provide a foundation for the researches on the cytologic classification, the molecular breeding, the cultivation of new variety and the cytological diagnosis of genetic transformation, which are related to Saintpaulia ionantha.Through comparing the various preprocessing methods and the effects of reagents, a conclusion was come to that the processing by 0.05% colchicine in 4 ~C for 2 h is the best. Results show that the Saintpaulia ionantha in this study is tetraploid, it has 8 long chromosome, 4 mid-long chromosome, 8 mid-short chromosome and 8 short chromosome. Karyotype belongs to 2B type, and the asymmetrical karyotype coefficient is 63.3% and the karyotype formula is 2n=4x=28= 16 m＋8 sm＋4 T. |
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| Keywords: | karyotype analysis Saintpaulia ionantha technology of tabletting of chromosome taking off cliff and low pressure seeping colchicine |
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