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| 内蒙古地区天然臭柏种群遗传多样性的RAPD分析 | |
| 引用本文： | 张国盛,张小红,王林和,梁小荣,温国胜,红雨.内蒙古地区天然臭柏种群遗传多样性的RAPD分析[J].干旱区资源与环境,2005,19(Z1):193-198. |
| 作者姓名： | 张国盛 张小红 王林和 梁小荣 温国胜 红雨 |
| 作者单位： | 1. 内蒙古农业大学,呼和浩特,010019 2. 北京世经未来投资咨询有限公司,100052 3. 浙江林学院,临安,311300 4. 内蒙古师范大学生命科学与技术学院,呼和浩特,010022 |
| 基金项目： | 国家自然科学基金项目(30271047);科委部重大基础研究前期研究专项(2004CCA03000). |
| 摘 要： | 应用随机扩增多态性DNA(RAPD)标记法研究了内蒙古地区臭柏种群的遗传分化,所用的18条引物对4个臭柏种群的55个样本扩增出124个位点,其中多态位点100个,多态率达80.7%。种内的平均Nei's多样性指数和Shannon's多样性指数分别为0.286和0.428,种群内分别为0.232和0.347,基因多样性变化趋势为毛乌素沙地(0.258,0.386)>阴山山脉西部(0.258,0.376)>浑善达克沙地(0.231,0.345)>内蒙古贺兰山(0.184,0.281)。臭柏种群总的基因多样性(Ht=0.288),大于种群间的基因多样性(Hs=0.233)。种群间的遗传分化系数(Gst)为0.183,种群内的遗传变异占总遗传变异的81.7%。臭柏种群间的平均遗传距离为0.086,平均相似性系数为0.918,4个种群之间有比较相似的遗传多样性,由于地理隔离,种群间也存在分化。 |
| 关 键 词： | 臭柏 RAPD 遗传多样性 遗传分化 |
| 文章编号： | 1003-7578(2005)07-193-06 |
| 修稿时间： | 2005年10月15 |
RAPD Analysis on Genetic Differentiation of Sabina vulgaris Populations in Inner Mongolia Area |
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| ZHANG Guo-sheng,ZHANG Xiao-hong,WANG Linhe,LIANG Xiao-Rong,WEN Guo-sheng,HONG Yu.RAPD Analysis on Genetic Differentiation of Sabina vulgaris Populations in Inner Mongolia Area[J].Journal of Arid Land Resources and Environment,2005,19(Z1):193-198. | |
| Authors: | ZHANG Guo-sheng ZHANG Xiao-hong WANG Linhe LIANG Xiao-Rong WEN Guo-sheng HONG Yu |
| Institution: | ZHANG Guo-sheng~ |
| Abstract: | Genetic Differentiation of Sabina vulgarisPopulation in Inner Mongolia area was studied with Random Amplified Polymorphic DNA(RAPD) markers.After analyzed by Popgen32 Version1.31,the results were as follows.Through the amplification with 18 random primers,124 repeatable loci in which 100 were poly-morphic were detected.Percentage of polymorphic loci was 80.7%.Nei'sindex and Shannon'sindex of the gene diversitywere 0.286 and 0.428 in special,and were 0.232 and 0.347 within populations respectively.The genetic diversities of each population were Mu Us sandland(0.258,0.386)>Yinshan mountain(0.258,0.376)>Hunshandake sandland(0.231,0.345)>Helan mountain(0.184,0.281).The genetic differentiation was Gst=0.183.The genetic variation among populations was 18.3%,and within population was 77.63%.The average genetic distance among populations was 0.086 and Nei's genetic identity was 0.918.The genetic diversities in 4 populations were similar,but the genetic differentiation existed because of geographic isolation. |
| Keywords: | Sabina vulgaris RAPD genetic diversity genetic differentiation |
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