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锗载体导入人参培养细胞的研究*
引用本文:蒋晶,王敬文.锗载体导入人参培养细胞的研究*[J].林业科学研究,1993,6(4):456-461.
作者姓名:蒋晶  王敬文
作者单位:中国林业科学研究院亚热带林业研究所 助理研究员,浙江 富阳 311400
摘    要:

关 键 词:人参    载体导入  细胞培养
收稿时间:1993/2/15 0:00:00

Study on Introduction of Germanium into Panax ginseng Cultured Cells via Chemical Vectors
Jiang Jing and Wang Jingwen.Study on Introduction of Germanium into Panax ginseng Cultured Cells via Chemical Vectors[J].Forest Research,1993,6(4):456-461.
Authors:Jiang Jing and Wang Jingwen
Institution:The Research Institute of Subtropical Forestry, CAF Fnyang, Zhejiang 311400;The Research Institute of Subtropical Forestry, CAF Fnyang, Zhejiang 311400
Abstract:A great quantity of germanium was abounded in wild Panax ginseng during its growing period for decades, and its medical effects were very strong. The content of germanium absorbed and accumulated in Panax ginseng cultured cells was about 8~17 ppm. Chemical vector compounds VA, VB, and VC of free toxicity for cells were prepared, and germanium was introduced into Panax ginseng cultured cells through using these vectors. During a growth period of 28 days, the quantity of germanium introduced via VA, VB, or VC attained upto 2978, 286, and 483 ppm respectively. Abound of germanium in Panax ginseng was a function of mature cells. Su-peraddition of germanium via vectors was reasonable at the end of logarithm period. Superaddition of germanium in culture had no influence on biosynthesis and accumulation of Panax ginseng saponin.
Keywords:Panax ginseng  germanium  vector introduction
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