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杜仲叶中绿原酸醇提法的工艺研究
引用本文:兰小艳,黄 敏,张学俊.杜仲叶中绿原酸醇提法的工艺研究[J].中国农学通报,2009,25(18):84-88.
作者姓名:兰小艳  黄 敏  张学俊
作者单位:1. 贵州省发酵工程及生物制药实验室,贵州大学,贵阳,550003;贵州大学化学工程学院,贵阳,550003
2. 贵州大学明德学院,贵阳,550003
3. 贵州省发酵工程及生物制药实验室,贵州大学,贵阳,550003
基金项目:贵州省科技厅"杜仲叶化学成分研究",贵州大学"博士基金"项目 
摘    要:目的 研究以乙醇为溶剂提取杜仲叶中绿原酸的最佳工艺条件。方法 通过单因素实验研究各因素对提取率的影响,应用正交实验优化工艺条件。结果 乙醇提取杜仲叶绿原酸最佳工艺条件为: 乙醇浓度为60%,浸提温度为60℃,时间为2.5h,物料比1:12。结论 在最佳条件下,绿原酸提取率为2.434%。

关 键 词:郁南无核黄皮  郁南无核黄皮  染色体  非整倍体  混倍体  
收稿时间:2009-05-12
修稿时间:2009-06-26

Study on the Alcohol Extraction Technology of Chlorogenic Acid from the Leaf of Eucommia ulmoides Oliv.
Lan Xiaoyan,Huang Ming,Zhang Xuejun.Study on the Alcohol Extraction Technology of Chlorogenic Acid from the Leaf of Eucommia ulmoides Oliv.[J].Chinese Agricultural Science Bulletin,2009,25(18):84-88.
Authors:Lan Xiaoyan  Huang Ming  Zhang Xuejun
Institution:Lan Xiaoyan, Huang Ming, Zhang Xuejun(1.Guizhou Province Key Laboratory of Fermentationt Engineering & Biological Pharmacy,Guizhou University, Guiyang 550003;2.Institute of Chemical Engineering, Guizhou University, Guiyang 550003;3.Intstitute of Mingde, Guizhou University, Guiyang 550003)
Abstract:Objective The extraction technology of chlorogenic acid from Eucommia ulmoides leaf was studied with Ethanol as solvent. Methods Main factors influencing the extraction of chlorogenic acid from the leaves of Eucommia ulmoides were studied by single factor experiments, and then the optimized conditions for the extraction of chlorogenic acid were studied by orthogonal test.Results Optimized conditions were obtained as follows: Ethanol concentration 60%;extraction temperature 60℃; extraction time 2.5h; material to solvent 1:12. Conclusion The extraction ratio of chlorogenic acid was 2.434% in the optimized conditions.
Keywords:Chlorogenic acidzz  Extraction  Leaf of Eucommia ulmoides  Ethanolzz
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