| 盐胁迫下转甜菜碱醛脱氢酶基因水稻幼苗耐盐生理的研究 |
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| 引用本文： | 孙耀中,东方阳,陈受宜,杨晓玲,刘永军,郭学民.盐胁迫下转甜菜碱醛脱氢酶基因水稻幼苗耐盐生理的研究[J].华北农学报,2004,19(3):38-42. |
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| 作者姓名： | 孙耀中 东方阳 陈受宜 杨晓玲 刘永军 郭学民 |
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| 作者单位： | 1. 河北科技师范学院,生命科学系,河北,昌黎,066600
2. 中国科学院遗传研究所,北京,100000 |
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| 基金项目： | 河北省秦皇岛市科学技术局重点资助课题(2002-04) |
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| 摘 要： | 在NaCl浓度0，3．0，5．0，7．0g／L下，对转甜菜碱醛脱氢酶(BADH)基因水稻的两个品系51—22，52—7及其受体亲本中花8号进行幼苗耐盐性试验。结果表明：转BADH基因水稻比其受体亲本耐盐性强，在高NaCl浓度(5．0，7．0g／L)胁迫下，甜菜碱醛脱氢酶基因可以提高盐胁迫下水稻幼苗过氧化氢酶(CAT)活性，提高根系活力和叶绿素含量，稳定细胞膜的透性，降低植株体内Na^ 的积累，从而减轻幼苗受盐害的程度。盐胁迫下水稻幼苗的CAT活性、SNa^ ／K^ 、叶Na^ ／K^ 、叶绿素含量是影响幼苗生长的主要生理指标。
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| 关 键 词： | 盐胁迫 甜菜碱醛脱氢酶基因 转基因水稻 幼苗 耐盐生理 |
| 文章编号： | 1000-7091(2004)02-0038-05 |
Study on the Physiology of Transgenic Rice Seedlings with Gene of Betaine Aldehyde Dehydrogenase Gene under Salt Stress |
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| SUN Yao-zhong,DONG Fang-yang,CHEN Shou-yi,YANG Xiao-ling,LIU Yong-jun,GUO Xue-min.Study on the Physiology of Transgenic Rice Seedlings with Gene of Betaine Aldehyde Dehydrogenase Gene under Salt Stress[J].Acta Agriculturae Boreali-Sinica,2004,19(3):38-42. |
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| Authors: | SUN Yao-zhong DONG Fang-yang CHEN Shou-yi YANG Xiao-ling LIU Yong-jun GUO Xue-min |
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| Abstract: | Salt stress experiments of rice seedlings of two lines with a gene encoding for BADH,51 - 22,52 -7 and the recipient Zhonghua No. 8, were conducted under the NaCl concentration of 0,3.0,5.0,7.0 g/L. The results showed that the rice cultivars with a gene encoding for BADH demonstrated higher salt tolerance than its recipient; The gene encoding for BADH could reduce the salt injury by increasing CAT activity, root activity, chlorophyll content and cell membrane osmosis,and by decreasing the Na+ concentration in the seedlings under high NaCl stress (NaCl concentration:5.0,7.0 g/L) ;The CAT activity, SNa*/K+ and chlorophyll content are the main physiological indexes affecting the seedling growth. |
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| Keywords: | Rice Betaine aldehyde dehydrogenase Salt stress Salt tolerance physiology |
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