Development of two microsatellite multiplex PCR systems for high throughput genotyping in Populus euphratica |
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| Authors: | Pascal Eusemann Steffen Fehrenz Martin Schnittler |
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| Institution: | (1) Department of Botany and Landscape Ecology, EMAU Greifswald, Grimmer Str. 88, 17487 Greifswald, Germany |
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| Abstract: | Eighteen microsatellite primer pairs previously developed at Oak Ridge National Laboratory for Populus tremuloides Michx. and Populus trichocarpa Torr. & Gray were screened for amplification in Euphrates poplar, Populus euphratica Oliv. Thirteen loci were found to express polymorphisms ranging from two to 17 alleles. The eight most variable loci were
selected to set up and optimize two multiplex polymerase chain reaction (PCR) assays. Three populations containing altogether
436 trees were used to characterize the selected loci and ascertain their applicability for parentage analysis and genotyping
studies. Through cross-checking of clonal identity against sex of the genotyped trees we estimated the maximum error rate
for merging genotypes to be less than 0.045.
Foundation project: This study was supported by the Deutsche Forschungsgemeinschaft DFG (grant number SCHN 1080/1-1) |
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| Keywords: | clone identification Euphrates poplar genetic fingerprint parentage analysis population structure SSR primers |
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