| 广西地方葛根种质资源遗传多样性的SCoT分析 |
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| 引用本文： | 尚小红,严华兵,曹升,肖亮,王艳,欧昆鹏.广西地方葛根种质资源遗传多样性的SCoT分析[J].核农学报,2019,33(7):1311-1317. |
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| 作者姓名： | 尚小红 严华兵 曹升 肖亮 王艳 欧昆鹏 |
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| 作者单位： | 广西壮族自治区农业科学院经济作物研究所,广西南宁530007;广西作物遗传改良生物技术重点开放实验室,广西南宁530007;广西壮族自治区农业科学院经济作物研究所,广西南宁,530007;广西作物遗传改良生物技术重点开放实验室,广西南宁,530007 |
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| 基金项目： | 广西重点研发计划(桂科AB16380087),广西藤县粉葛试验站(TS201431),广西科技基地和人才专项(桂科AD17195072) |
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| 摘 要： | 为了解广西地方葛根种质资源之间的亲缘关系,本研究以SCoT分子标记对44份广西葛根种质资源的基因组DNA进行PCR扩增,并进行遗传多样性分析。结果表明,25条SCoT引物共扩增出223个条带,其中具有多态性的条带有194条,平均每条引物获得7.76个多态性条带,多态性比例为86.99%。聚类分析表明,44份葛根种质资源的遗传相似系数在0.587~0.982之间。在系数0.65处,44份葛根分为两大类,37号材料单独成为一类;在系数为0.74处,可聚为六类。综上所述,SCoT分子标记适用于葛根种质资源遗传多样性分析,本研究结果为广西葛根种质资源鉴定评价和新品种选育奠定了一定的理论基础。
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| 关 键 词： | 葛根 广西地方品种 SCoT 遗传多样性分析 |
| 收稿时间： | 2018-03-02 |
Genetic Diversity Analysis of Pueraria in Guangxi Based on SCoT Markers |
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| SHANG Xiaohong,YAN Huabing,CAO Sheng,XIAO Liang,WANG Yan,OU Kunpeng.Genetic Diversity Analysis of Pueraria in Guangxi Based on SCoT Markers[J].Acta Agriculturae Nucleatae Sinica,2019,33(7):1311-1317. |
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| Authors: | SHANG Xiaohong YAN Huabing CAO Sheng XIAO Liang WANG Yan OU Kunpeng |
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| Institution: | 1 Cash Crops Research Institute,Guangxi Academy of Agricultural Sciences, Nanning, Guangxi 530007;2 Guangxi Crop Genetic Improvement and Biotechnology Key Lab, Nanning, Guangxi 530007 |
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| Abstract: | In order to analyze the genetic similarity of 44 Pueraria germplasm resources in Guangxi, the DNA of the germplasm resources was amplified by SCoT primers.The results showed that, 25 SCoT primers produced 233 bands, among which 194 bands were polymorphic, the percentage of polymorphic rate was up to 86.99%, and the average polymorphic number was 7.76 per primer. The cluster analysis showed that the range of genetic similarity(GS)was 0.587 to 0.982 among 44 Pueraria samples,the samples could be divided into 2 groups at the GS of 0.65, the 37th germplasm alone was clustered into one group, and the rest accessions was clustered into another group. All of the accessions could be clustered into six groups at the GS of 0.74. The result revealed that SCoT molecular markers are suitable for the analysis of genetic diversity of the Pueraria germplasm resources, and will lay a foundation for the evaluation and selection of the germplasm resources of Pueraria in Guangxi. |
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| Keywords: | Pueraria Guangxi landraces SCoT genetic diversity |
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