| 冬麦免耕覆盖栽培对土壤丛枝菌根真菌多样性的影响 |
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| 引用本文： | 汪志琴,马琨,王小玲,李越,魏常慧.冬麦免耕覆盖栽培对土壤丛枝菌根真菌多样性的影响[J].核农学报,2019,33(5):969-977. |
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| 作者姓名： | 汪志琴 马琨 王小玲 李越 魏常慧 |
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| 作者单位： | 宁夏大学农学院,宁夏回族自治区银川750021;宁夏大学西北土地退化与生态恢复国家重点实验室培育基地,宁夏回族自治区银川750021;宁夏大学西北土地退化与生态恢复国家重点实验室培育基地,宁夏回族自治区银川,750021 |
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| 基金项目： | 国家自然科学基金(31660132、31160104),宁夏高等学校一流学科建设项目(NXYLXK2017B06) |
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| 摘 要： | 为揭示冬麦免耕覆盖栽培管理措施对土壤丛枝菌根真菌多样性的影响,以农田土壤生态系统为研究对象,利用Illumina MiSeq平台通过高通量测序的方法,对连续免耕覆盖栽培及有机肥管理下冬麦土壤丛枝菌根真菌群落组成及其与土壤环境因子间的相互关系进行研究。结果表明,丛枝菌根(AM)真菌在97%相似度下共获得4 515个AM真菌的操作分类单元(OTUs),分属于1门3纲4目8科10属155种。不同处理中,类球囊霉属(Paraglomus),球囊霉属(Glomus)和近明球囊霉(Claroideoglomus)为优势属,受农业综合管理措施中有机肥施用、免耕覆盖因子的影响,近明球囊霉、有隔球囊霉属相对丰度在不同处理间存在显著差异(P<0.05)。与耕作、不覆盖相比,免耕、覆盖措施提高了AM真菌多样性指数,但降低了AM真菌属的丰富度;与无肥条件相比,有机肥施用提高了AM真菌丰富度指数,但降低了AM真菌多样性指数。多元分析结果表明,土壤全磷及速效磷含量是影响AM真菌群落组成中优势属丰度变化的主要因素。免耕覆盖、有机栽培改变了土壤AM真菌的多样性及丰富度,土壤中丰度较低的AM真菌菌属更容易受到有机肥施用的影响;有机肥与免耕、覆盖管理的交互作用对AM真菌近明球囊霉属相对丰度有显著影响(P<0.05)。AM真菌群落对耕作覆盖、有机肥施用管理活动的响应受到农业综合管理活动及其相关因素交互作用的影响。本研究结果为合理农作物免耕覆盖、有机栽培管理提供了理论依据。
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| 关 键 词： | 免耕覆盖 丛植菌根真菌 多样性 丰富度 高通量测序 |
| 收稿时间： | 2018-06-20 |
Effect of No-tillage and Mulching Management on Soil Arbuscular Mycorrhizal Fungal Diversity |
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| WANG Zhiqin,MA Kun,WANG Xiaoling,LI Yue,WEI Changhui.Effect of No-tillage and Mulching Management on Soil Arbuscular Mycorrhizal Fungal Diversity[J].Acta Agriculturae Nucleatae Sinica,2019,33(5):969-977. |
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| Authors: | WANG Zhiqin MA Kun WANG Xiaoling LI Yue WEI Changhui |
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| Institution: | 1 School of Agriculture, Ningxia University, Yinchuan, Ningxia Hui Autonomous Region 750021;2 Breeding Base for State Key Laboratory of Land Degradation and Ecological Restoration in Northwestern China, Ningxia University, Yinchuan, Ningxia Hui Autonomous Region 750021 |
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| Abstract: | Arbuscular mycorrhizal fungi play a crucial role in maintaining the sustainability and stability of the soil ecosystem. In order to reveal the influence of no-tillage with mulching and organic fertilization on the diversity of soil arbuscular mycorrhizal(AM) fungi after the continuous winter wheat farming for two years, the relationship between the arbuscular mycorrhizal fungal community structure and soil environmental factors under the continuous no-tillage mulching and fertilization management were studied by using illumina miseq platform through high-throughput sequencing method. A total of 4 515 operational taxa (OTUs) of AM fungi were obtained under 97% similarity, belonging to 155 species, 1 phylum, 3 classes, 4 orders, 8 families, 10 genera. Paraglomus, Glomus and Clarithroides were the dominant genera in all treatments. Under the influence of organic fertilizer and no-tillage mulching factors, the relative abundance of Claroideoglomus and Septoglomus were significantly difference among different treatments (P<0.05). Compared with tillage and non-mulching, no-tillage and mulching measures increased the diversity index of AM fungi, but the abundance of AM fungi were decreased under the condition of organic fertilizer, compared with no fertilizer condition. The application of organic fertilizer increased AM fungi richness index, but decreased AM fungi diversity index. The results of multivariate analysis showed that the contents of total phosphorus and the available phosphorus in soil were the main influence factors to the change of the dominant genera abundance of AM fungal communities. The diversity and the abundance of AM fungi were changed by no-tillage mulching and organic fertilization. AM fungi genera with lower abundance in soil, are more susceptible to the influence by organic fertilization management. The interaction of organic fertilization with no-tillage and mulching management had a significant effect on the relative abundance of Claroideoglomus. The response of AM fungal community to tillage, mulch and the organic fertilization management measures are influenced by multiple factors of agriculture management and also influenced by complex interactions with management factors with each other. This study offers a theoretic basis for rational crop no-tillage with mulching and organic cultivation management in the mountain areas of south Ningxia. |
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| Keywords: | no-tillage and mulching arbuscular mycorrhizal fungi diversity richness high throughput sequencing |
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