| 冬瓜实时荧光定量PCR内参基因的筛选与评价 |
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| 引用本文： | 叶新如,朱海生,林珲,刘建汀,王彬,陈敏氡,温庆放.冬瓜实时荧光定量PCR内参基因的筛选与评价[J].核农学报,2019,33(3):473-481. |
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| 作者姓名： | 叶新如 朱海生 林珲 刘建汀 王彬 陈敏氡 温庆放 |
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| 作者单位： | 福建省农业科学院作物研究所/福建省农业科学院蔬菜研究中心/福建省蔬菜工程技术研究中心,福建 福州 350013 |
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| 基金项目： | 福建省属公益类科研院所基本科研专项(2018R1026-2), 福建省农业科学院蔬菜科技创新团队(STI2017-1-2),福建省农业科学院“青年科技英才百人计划”(YC2017-5),中央引导地方科技发展专项(2018L3005) |
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| 摘 要： | 为筛选适合冬瓜稳定表达的内参基因,以黑皮冬瓜低温、高温、干旱胁迫处理不同时间的叶片和正常处理不同组织为试验材料,利用RT-qPCR技术,结合GeNorm、NormFinder和BestKeeper软件评价7个候选内参基因(28SrRNA、UBQ、RP Ⅱ、GAPDH、EF-1α、UBC21和TUA)稳定性。结果表明,7个候选内参基因在不同组织和不同胁迫处理下表达丰度及稳定性存在差异; EF-1α在低温胁迫处理下表达稳定性最好;UBQ和TUA在高温和干旱胁迫处理下表达稳定性最好; EF-1α 在不同组织中表达稳定性最好。本研究结果为冬瓜内参基因的选择提供了一定的理论参考。
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| 关 键 词： | 冬瓜 内参基因 非生物胁迫 |
| 收稿时间： | 2018-07-31 |
Screening and Evaluation of Reference Genes for RT-qPCR in Wax Gourd (Benincasa hispida Cogn.) |
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| YE Xinru,ZHU Haisheng,LIN Hui,LIU Jiangting,WANG Bin,CHEN Mindong,WEN Qingfang.Screening and Evaluation of Reference Genes for RT-qPCR in Wax Gourd (Benincasa hispida Cogn.)[J].Acta Agriculturae Nucleatae Sinica,2019,33(3):473-481. |
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| Authors: | YE Xinru ZHU Haisheng LIN Hui LIU Jiangting WANG Bin CHEN Mindong WEN Qingfang |
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| Institution: | Fujian Engineering Research Center for Vegetables/Vegetable Research Center, Fujian Academy of Agricultural Sciences/Crops Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350013 |
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| Abstract: | The present study aimed at selecting the stable reference genes to ensure the reliability and accuracy in gene expression analysis of wax gourd (Benincasa hispida Cogn.). By RT-PCR technology, combined with GeNorm, NormFinder, BestKeeper and ReFinder analysis, we investigated the expression stability of seven candidate reference genes(28SrRNA、UBQ、RP Ⅱ、GAPDH、EF-1α、UBC21、TUA). The results showed that, in different tissues and with different stress treatment,the expression abundance and stability of these seven reference genes were different. The expression stability of EF-1α was the best under low temperature stress., UBQ and TUA showed the best expression stability under high temperature and drought stress, EF-1α was the best reference gene for different tissues. The results of this study can be used as a reference for the selection of internal reference genes in wax gourd. |
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| Keywords: | wax gourd reference gene abiotic stress |
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