| 干旱胁迫下H2S对板栗幼苗根系抗氧化特性及呼吸相关酶活性的影响 |
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| 引用本文： | 孙晓莉,田寿乐,沈广宁,姜倩倩,许林.干旱胁迫下H2S对板栗幼苗根系抗氧化特性及呼吸相关酶活性的影响[J].核农学报,2019,33(5):1024-1031. |
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| 作者姓名： | 孙晓莉 田寿乐 沈广宁 姜倩倩 许林 |
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| 作者单位： | 1 山东省果树研究所,山东 泰安 271000; 2 潍坊学院生物与农业工程学院,山东 潍坊 261061 |
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| 基金项目： | 山东省农业科学院青年科研基金资助项目(2016YQN29),山东省农业良种工程项目(2016LZGC012),山东省农业科学院农业科技创新工程项目(CXGC2016A03) |
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| 摘 要： | 为探究干旱胁迫下H2S对板栗幼苗根系特性的影响,以黄棚板栗幼苗为试验材料,通过PEG模拟干旱并进行H2S供体硫氢化钠(NaHS)和清除剂次牛磺酸(HT)处理,分析板栗幼苗根系超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)等抗氧化酶活性及丙二醛(MDA)、H2O2等抗氧化物质含量,检测苹果酸脱氢酶(MDH)、磷酸果糖激酶(PFK)和葡萄糖-6-磷酸脱氢酶(G-6-PDH)等呼吸代谢相关酶活性。结果表明,0.5 mmol·L-1 NaHS+15% PEG协同处理24 h后,与15% PEG处理相比,根系SOD、POD、CAT、APX活性分别提高19.64%、24.67%、56.91%、16.29%,根系脯氨酸含量增加15.25%,根系MDA和H2O2含量分别降低26.52%和20.86%,MDH、PFK和G-6-PDH活性分别提高27.28%、23.14%和15.36%;而添加H2S清除剂HT则逆转了上述变化,说明适宜浓度的NaHS能够减轻干旱胁迫对板栗根系膜脂过氧化伤害和对呼吸代谢的抑制,从而有利于提高板栗幼苗对干旱逆境的适应能力。本研究结果为进一步探索H2S缓解板栗树干旱胁迫的机理提供了理论依据。
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| 关 键 词： | 硫化氢 干旱胁迫 抗氧化 呼吸代谢 |
| 收稿时间： | 2017-10-30 |
Effect of Exogenous Hydrogen Sulfide on Antioxidant Characteristics and Respiratory Related Enzymes in Root of Chestnut Seedlings Under Drought Stress |
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| SUN Xiaoli,TIAN Shoule,SHEN Guangning,JIANG Qianqian,XU Lin.Effect of Exogenous Hydrogen Sulfide on Antioxidant Characteristics and Respiratory Related Enzymes in Root of Chestnut Seedlings Under Drought Stress[J].Acta Agriculturae Nucleatae Sinica,2019,33(5):1024-1031. |
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| Authors: | SUN Xiaoli TIAN Shoule SHEN Guangning JIANG Qianqian XU Lin |
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| Institution: | 1 Shandong Institute of Pomology, Tai'an, Shandong 271000;2 College of Biological and Agricultural Engineering, Weifang University, Weifang, Shandong 261061 |
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| Abstract: | In order to investigate effect of H2S on root characteristics of chestnut (Castanea mollissima) seedlings under drought stress, the chestnut seedlings of Huangpeng were used as experimental material, the antioxidant enzyme (SOD, POD, CAT, APX) activities, the content of MDA and H2O2, and the respiration metabolism enzymes (MDH, PFK, G-6-PDH) activities were analyzed under drought stress induced by 15% PEG and treated with the H2S donor sodium hydrosulfide (NaHS) and scavenger hypothiosulfate (HT) in this study. The results showed that under the treatment of 0.5 mmol·L-1 NaHS+15% PEG for 24 h, compared to 15% PEG treatment, the activity of SOD, POD, CAT and APX in root was increased by 19.64%, 24.67%, 56.91% and 16.29%, respectively; the proline content increased by 15.25%; the contents of MDA and H2O2 were significantly decreased by 26.52% and 20.86%, respectively; and the activities of MDH, PFK and G-6-PDH were significantly increased by 27.28%, 23.14%, 15.36%, respectively. However, the addition of H2S scavenger HT reversed the above effects. The results showed that appropriate concentration of exogenous H2S was favorable to reduce membrane lipid peroxidation damage and alleviate inhibition of respiratory metabolism in the root of chestnut seedlings under drought stress, thus improving the adaptability of chestnut seedlings to drought stress. This study provided a theoretical basis for further exploring the mechanism of H2S alleviating drought stress in chestnut trees. |
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| Keywords: | hydrogen sulfide drought stress antioxidant respiratory metabolism |
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