| 盐胁迫下草麻黄电阻抗图谱参数的变化及离子平衡机制 |
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| 引用本文： | 张丽,牛雪飞,郭栋,张艳阳,贾志国,刘俊喜,袁卉馥.盐胁迫下草麻黄电阻抗图谱参数的变化及离子平衡机制[J].核农学报,2019,33(6):1208-1216. |
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| 作者姓名： | 张丽 牛雪飞 郭栋 张艳阳 贾志国 刘俊喜 袁卉馥 |
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| 作者单位： | 河北北方学院,河北张家口,075000;河北北方学院,河北张家口,075000;河北北方学院,河北张家口,075000;河北北方学院,河北张家口,075000;河北北方学院,河北张家口,075000;河北北方学院,河北张家口,075000;河北北方学院,河北张家口,075000 |
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| 基金项目： | 河北北方学院自然科学研究计划重大项目“冀西北坝上地区麻黄生产及防风固沙研究”(ZD201408),河北省科技厅课题“冀西北道地药材麻黄、金莲花优质高产栽培技术体系研究”(16232503D-X-13) |
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| 摘 要： | 为探讨草麻黄对盐胁迫的适应性,以2年生草麻黄苗为试验材料,采用盆栽法测定在不同NaCl浓度、不同胁迫时间下草麻黄苗各项电阻抗图谱参数(EIS)变化及5种矿质离子含量,并分析各电阻抗参数、相对电导率及矿质离子含量之间的相关性。结果表明,随着NaCl浓度的增加和胁迫时间的延长,草麻黄地上部相对电导率逐渐升高,其中200 mmol·L-1NaCl胁迫28 d以上时的相对电导率显著高于对照,200、300、400 mmol·L-1盐胁迫下的相对电导率分别为对照的1.43、1.52、1.65倍;电阻抗图谱参数高频电阻率(r)、低频电阻率(r1)、胞内电阻率(ri)、胞外电阻率(re)、弛豫时间(τ)及弛豫时间分布系数(Ψ)均呈先下降后上升再下降或先下降后上升的变化趋势,其中r、r1、re、ri、Ψ均在NaCl浓度超过200 mmol·L-1时出现再次下降,且呈不可逆的下降趋势。随着胁迫天数的增加,草麻黄地上部和根中Na+含量逐渐增加,K+含量逐渐下降,K+/Na+显著降低。盐胁迫下,草麻黄地上部Zn、Fe、Mg含量均有不同程度的降低。相关性分析结果表明,K+/Na+与re、r、r1均呈极显著正相关,与ri呈显著正相关,其中 K+/Na+ 与re的相关性最高,相关系数为0.985;5种阳离子总量与re、r、r1均呈显著负相关。盐胁迫影响了草麻黄体内离子的运输和平衡,细胞内外离子浓度的变化引发电阻抗参数的变化,分析相对电导率电阻抗图谱参数的变化发现NaCl胁迫下,草麻黄的忍耐极限浓度为200 mmol·L-1,表明草麻黄具有较高的耐盐能力。本研究结果为盐碱地区草麻黄的推广栽培提供了理论依据。
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| 关 键 词： | 草麻黄 盐胁迫 电阻抗图谱 离子平衡 |
| 收稿时间： | 2018-01-15 |
Changes of Electrical Impedance Parameters and Ion Balance Mechanism to Salt Stress of Ephedra sinica Stapf |
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| ZHANG Li,NIU Xuefei,GUO Dong,ZHANG Yanyang,JIA Zhiguo,LIU Junxi,YUAN Huifu.Changes of Electrical Impedance Parameters and Ion Balance Mechanism to Salt Stress of Ephedra sinica Stapf[J].Acta Agriculturae Nucleatae Sinica,2019,33(6):1208-1216. |
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| Authors: | ZHANG Li NIU Xuefei GUO Dong ZHANG Yanyang JIA Zhiguo LIU Junxi YUAN Huifu |
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| Institution: | Hebei North University, Zhangjiakou, Hebei 075000 |
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| Abstract: | In order to study the adaptability of Ephedra sinica stapf on salt stress, 2 year-old seedlings of Ephedra sinica stapf were used as the test materials, a pot experiment was conducted to determine the electrical impedance spectroscopy(EIS) parameters and five mineral ions contents of different NaCl concentration and stress time. The correlations were analyzed among the relative conductivity, the electrical impedance spectroscopy(EIS) parameters and mineral ion contents.The results showed that with the increasing concentrations and the extension stress time of NaCl, the relative conductivity increased gradually and was higher than the control under 200 mmol·L-1 NaCl stress for more than 28 days, which was 1.43, 1.52, 1.65 times of the control under 200, 300, 400 mmol·L-1 NaCl stress, Specific resistance in high frequency (r), specific resistance in low frequency (r1), intracellular resistance (ri), extracellular resistance (re), relaxation time (τ) and distribution coefficient of relaxation time (Ψ) decreased firstly, then increased, and then finally decreased again or decreased firstly, then increased in shoots of Ephedra sinica stapf. The second decline turning point of r, r1, re, ri and Ψ occurred at the concentration above 200 mmol·L-1NaCl, showing an irreversible downward trend. The contents of Na+ increased gradually with prolongation of stress time in shoots and roots while the contents of K+decreased, so the K+/Na+ ratio declined significantly. In addition, the contents of Zn, Fe and Mg decreased at different degrees to salt stress in shoots. The correlation analysis indicated that very significant positive correlation was found between the K+/Na+ ratio and four EIS parameters re, ri, and r1, the significant positive correlation was found between the K+/Na+ ratio and r. The correlation between K+/Na+ ratio and re was the highest, the correlation coefficient was 0.985; The significantly negative correlation was found between sum of five cations and re, r, r1. Salt stress influenced the transport and balance of ion in Ephedra sinica stapf, the changes of electrical impedance spectroscopy parameters were produced by changes of ion concentrations in cell. Through analyzing the changesg of EIS and the relative conductivity, Ephedra sinica stapf might be tolerant to NaCl stress of 200 mmol·L-1. Ephedra sinica stapf could tolerate higher concentration of salt stress. The results provided a basis for extending culture of Ephedra sinica in saline-alkali areas. |
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| Keywords: | Ephedra sinica Stapf salt stress electrical impedance spectroscopy ion balance |
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