| 大麦白粉病抗性的遗传分析与QTL定位 |
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| 引用本文： | 费新茹,朱娟,郭晖,郭红,吕超,郭宝健,许如根.大麦白粉病抗性的遗传分析与QTL定位[J].核农学报,2019,33(5):888-893. |
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| 作者姓名： | 费新茹 朱娟 郭晖 郭红 吕超 郭宝健 许如根 |
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| 作者单位： | 扬州大学大麦研究所/江苏省作物遗传生理国家重点实验室/粮食作物现代产业技术协同创新中心/教育部植物功能基因组学重点实验室,江苏扬州225009;盐都区农业科学研究所,江苏盐城224011;扬州大学大麦研究所/江苏省作物遗传生理国家重点实验室/粮食作物现代产业技术协同创新中心/教育部植物功能基因组学重点实验室,江苏扬州,225009;江苏省农垦农业发展股份有限公司,江苏南京,210008;盐都区农业科学研究所,江苏盐城,224011 |
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| 基金项目： | 国家重点研发计划项目(2018YFD1000703、2018YFD1000700),国家大麦青稞产业技术体系建设专项(CARS-05),江苏高校优势学科建设工程项目 |
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| 摘 要： | 为探究大麦白粉病抗性遗传,定位其抗性QTL,本研究以抗病品种Gairdner和感病品种扬饲麦1号杂交F1花药培养构建的DH群体及亲本为材料,对大麦白粉病抗性进行鉴定与遗传分析,并利用91对在亲本间多态性好的SSR标记构建了群体的遗传连锁图谱,采用Windows QTL IciMapping 4.0软件中的完备区间-加性模型对大麦白粉病抗性QTL进行定位。结果表明,DH群体各系间存在丰富的大麦白粉病抗性遗传变异。共检测到5个与大麦白粉病抗性相关的QTLs。其中3个时期均检测到qPM-2Ha位于Bmag0711-AWBMS56区间,可解释的表型变异为7.48%~12.50%;qPM-4Ha位于EBmac0906-HVM68区间,可解释的表型变异为23.07%~32.09%;2个时期均检测到qPM-2Hb位于Bmag0749-GBM1475区间,可解释的表型变异为6.22%~8.13%。qPM-2Ha、qPM-4Ha及qPM-2Hb白粉病抗性基因均来源于抗病亲本Gairdner, qPM-3Ha和qPM-4Hb白粉病抗性基因来源于感病亲本扬饲麦1号,qPM-2Hb和qPM-3Ha可能是2个新的大麦白粉病抗性QTLs位点。本研究结果为大麦白粉病抗性基因的发掘、精细定位、克隆及分子标记辅助选择育种奠定了基础。
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| 关 键 词： | 大麦 DH群体 白粉病 QTL定位 |
| 收稿时间： | 2018-01-05 |
Genetic Analysis and QTL Mapping of Resistance to Powdery Mildew in Barley(Hordeum vulgare L.) |
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| FEI Xinru,ZHU Juan,GUO Hui,GUO Hong,LYU Chao,GUO Baojian,XU Rugen.Genetic Analysis and QTL Mapping of Resistance to Powdery Mildew in Barley(Hordeum vulgare L.)[J].Acta Agriculturae Nucleatae Sinica,2019,33(5):888-893. |
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| Authors: | FEI Xinru ZHU Juan GUO Hui GUO Hong LYU Chao GUO Baojian XU Rugen |
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| Institution: | 1 Key Laboratory of Plant Functional Genomics of the Ministry of Education/Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology/Barley Research Institution of Yangzhou University, Yangzhou, Jiangsu 225009;2 Yandu District Agricultural Science Research Institute, Yancheng, Jiangsu 224011;3 Jiangsu Provincial Agricultural Reclamation and Development Corporation, Nanjing, Jiangsu 210008 |
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| Abstract: | In order to explore the inheritance of resistance to barley powdery mildew and locate its resistance QTLs, in the present study, a total of 173 doubled-haploid (DH) lines generated from a cross between a disease-resistant cultivar Gairdner and a susceptible Chinese landrace barley Yangsimai 1 were used to identify the resistance to barley powdery mildew and andyzed its genetic characteristics. A genetic linkage map including 91 pairs of SSR markers with obvious polymorphism between the parents was constructed. QTL mapping for barley powdery mildew was carried out by using the complete interval-additive model (Inclusive Composite Interval-Additive Mapping, ICIM-ADD) method in Windows QTL IciMapping 4.0 software. The results showed that there were abundant genetic variations of barley powdery mildew resistance in the DH population. Five QTLs for the disease index of barley powdery mildew were identified, among which two QTLs (qPM-2Ha and qPM-4Ha ) were detected in all three stages. qPM-2Ha was located in the Bmag0711 ~ AWBMS56 interval, explained 7.48% ~ 12.50% of barley powdery mildew resistant variation; qPM-4Ha was located in the EBmac0906 ~ HVM68 interval, explained 23.07% ~ 32.09% of barley powdery mildew resistant variation; qPM-2Hb was detected in the first and second stages, located in the Bmag0749 ~ GBM1475 interval, explained 6.22% ~ 8.13% of barley powdery mildew resistant variation. qPM-2Ha,qPM-4Ha and qPM-2Hb that enhance the resistance to powdery mildew were derived from Gairdner, the resistant parent, while qPM-3Ha and qPM-4Hb were derived from the susceptible parent Yangsimai 1. Two putative QTLs, qPM-2Hb and qPM-3Ha likely represent new loci for barley powdery mildew resistance. The results have laid a foundation for further exploration, fine mapping, gene cloning and molecular-marker-assisted selection breeding of barley powdery mildew. |
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| Keywords: | barley DH population barley powdery mildew QTL mapping |
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