| 苯并芘(BaP)对鲤鱼肝、肾抗氧化、非特异性免疫能力及组织结构的影响 |
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| 引用本文： | 陈剑杰,曹谨玲,贺鑫晋,郭文静,罗永巨,高荣琨.苯并芘(BaP)对鲤鱼肝、肾抗氧化、非特异性免疫能力及组织结构的影响[J].核农学报,2019,33(3):623-630. |
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| 作者姓名： | 陈剑杰 曹谨玲 贺鑫晋 郭文静 罗永巨 高荣琨 |
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| 作者单位： | 山西农业大学动物科技学院/山西省生态畜牧与环境兽医学重点实验室,山西太谷,030801;广西水产研究所,广西南宁,530021 |
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| 基金项目： | 国家自然科学基金(31440087、31502141),中国博士后科学基金项目(2012M520601、2013T60267),山西农业大学博士后基金(92462) |
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| 摘 要： | 为探究苯并芘(BaP)对鲤鱼的毒性机制,本研究检测了不同浓度BaP(0、0.1、0.5、1.0 μg·L-1)暴露对鲤鱼肝、肾组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽(GSH)、丙二醛(MDA)及非特异性免疫酶溶菌酶(LZM)、酚氧化酶(PO)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性,以及硝基四氮唑兰(NBT)阳性细胞及肝、肾组织结构的影响。结果表明,1.0 μg·L-1 BaP暴露30 d后,鲤鱼肝、肾组织SOD活性、MDA含量及肝组织CAT、肾组织GSH活性均显著低于对照组;1.0 μg·L-1 BaP暴露的肝、肾组织LZM、AKP和PO活性以及肝组织ACP活性均显著低于对照组;不同浓度BaP暴露30 d后,鲤鱼NBT阳性细胞数均显著下降,且对肝、肾组织造成不同程度的损伤。相关性分析结果表明,BaP暴露浓度与肝、肾组织的SOD活性呈负相关,与MDA含量呈正相关,与肝、肾组织的AKP、ACP、PO活性呈负相关,与NBT阳性细胞数呈负相关。综上,水体中BaP暴露对鲤鱼肝、肾抗氧化和非特异性免疫能力及组织结构产生了一定程度的负面影响,本研究为水体中BaP的生态毒性效应研究提供了理论参考。
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| 关 键 词： | 肝、肾组织 抗氧化能力 非特异性免疫 组织结构 |
| 收稿时间： | 2017-06-14 |
Effects of Benzopyrene on the Capacity of Antioxidases,Non-specific Immunity and Organization Structure in Liver and Kidney of Carp (Cyprinus carpio) |
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| CHEN Jianjie,CAO Jinling,HE Xinjin,GUO Wenjing,LUO Yongju,GAO Rongkong.Effects of Benzopyrene on the Capacity of Antioxidases,Non-specific Immunity and Organization Structure in Liver and Kidney of Carp (Cyprinus carpio)[J].Acta Agriculturae Nucleatae Sinica,2019,33(3):623-630. |
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| Authors: | CHEN Jianjie CAO Jinling HE Xinjin GUO Wenjing LUO Yongju GAO Rongkong |
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| Institution: | 1 Key Lab of Ecological Animal Husbandry and Environmental Veterinary Medicine of Shanxi Province/College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi 030801; 2 Guangxi Academy of Fishery Sciences, Nanning, Guangxi 530021 |
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| Abstract: | To investigate the toxic mechanism of BaP on carp (Cyprinus carpio), the antioxidases (SOD, CAT, GSH) activities, MDA content, and the activities of non-specific immunity enzymes (LZM, PO, AKP, ACP), NBT positive cells and organization structure in liver and kidney of carp exposed to different concentrations of BaP (0, 0.1, 0.5, 1.0 μg·L-1) for 30 days were studied. The results showed that after exposure for 30 days, SOD activity and MDA content in liver and kidney, CAT activity in liver, and GSH activity in kidney exposed to 1.0 μg·L-1 of BaP were significantly lower than those in the control group. The activities of LZM, AKP and PO in liver and kidney, and ACP activity in liver exposed to 1.0 μg·L-1 of BaP were significantly decreased compared with control group. The number of NBT positive cells decreased significantly compared with control group. The liver and kidney tissue were damaged to different degrees. Correlation analysis indicated that the BaP concentration was negatively correlated with SOD activities, was positively correlated with MDA content in liver and kidney, had a negative correlation with the activities of AKP, ACP, PO in liver and kidney, was negatively correlated with the number of NBT positive cells. All above indicated that exposure to BaP in water could negatively affect the antioxidant ability, non-specific immunity function and organization structure in liver and kidney of carp to some extent, which provided the important information for further study of the ecological toxicity of BaP. |
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| Keywords: | liver and kidney antioxidant capacity non-specific immunity organization structure |
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