| 新疆巴州幼年与成年牦牛瘤胃细菌区系多样性分析 |
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| 引用本文： | 聂召龙,刘书杰,崔占鸿,潘浩,柴沙驼,孙璐,张晓卫,冯宇哲.新疆巴州幼年与成年牦牛瘤胃细菌区系多样性分析[J].核农学报,2019,33(11):2147-2157. |
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| 作者姓名： | 聂召龙 刘书杰 崔占鸿 潘浩 柴沙驼 孙璐 张晓卫 冯宇哲 |
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| 作者单位： | 青海大学畜牧兽医科学院/青海省牦牛工程技术研究中心/青海省高原放牧家畜动物营养与饲料科学重点实验室,青海 西宁 810016 |
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| 基金项目： | 青海省“高端创新人才千人计划”拔尖人才,中国科学院“西部青年学者项目”A类,教育部“春晖计划”合作科研项目(z2016095),青海省科技厅项目(2017-GX-G06) |
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| 摘 要： | 为比较年龄对牦牛瘤胃细菌区系多样性的影响,以新疆巴州1岁幼年牦牛和4岁成年牦牛的瘤胃微生物总DNA为研究材料,利用16S rRNA基因序列分析技术对牦牛瘤胃细菌区系的操作归类单元(OTU)聚类、门和属水平上的物种丰度、Alpha多样性进行比较分析。结果表明,在门水平上,厚壁菌门(Firmicutes)相对丰度为66.96%~70.31%,拟杆菌门(Bacteroides)相对丰度为21.87%~24.11%,其他菌类所占比例极少。在属水平上,普雷沃菌属(Prevotella)在幼年牦牛瘤胃内相对丰度(4.69%)高于成年牦牛(2.68%);瘤胃球菌属(Ruminococcus)在幼年牦牛瘤胃内相对丰度(4.69%)比在成年牦牛瘤胃内(1.79%)高。幼年和成年牦牛的Chao1指数分别为783.50和544.00,Shannon指数分别为4.68和4.52。综上可知,在同一草地放牧饲养条件下,1岁和4岁巴州牦牛瘤胃细菌区系组成存在明显差异,幼年牦牛瘤胃消化吸收的功能正在转化建立的过程中,所以Chao1指数和Shannon指数高于成年牦牛,且瘤胃细菌物种丰度和多样性更高。本研究结果为该地区牦牛瘤胃微生物稳态及其营养调控研究提供了参考依据。
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| 关 键 词： | 幼年牦牛 成年牦牛 瘤胃 细菌多样性 16SrRNA序列分析 |
| 收稿时间： | 2019-01-24 |
Analysis of Rumen Bacterial Flora Diversity in Young and Adult Bazhou Yaks in Xinjiang |
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| NIE Zhaolong,LIU Shujie,CUI Zhanhong,PAN Hao,CHAI Shatuo,SUN Lu,ZHANG Xiaowei,FENG Yuzhe.Analysis of Rumen Bacterial Flora Diversity in Young and Adult Bazhou Yaks in Xinjiang[J].Acta Agriculturae Nucleatae Sinica,2019,33(11):2147-2157. |
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| Authors: | NIE Zhaolong LIU Shujie CUI Zhanhong PAN Hao CHAI Shatuo SUN Lu ZHANG Xiaowei FENG Yuzhe |
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| Institution: | Qinghai Academy of Animal Husbandry and Veterinary Sciences in Qinghai University/Qinghai Yak Engineering Technology Research Center/Key Laboratory of Plateau Grazing Animal Nutrition and Feed Science of Qinghai Province, Xining, Qinghai 810016 |
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| Abstract: | Aimto study the effects of different age on rumen bacterial flora diversity in yaks. Total DNA of rumen microorganisms from 1-year-old young bazhou yaks and 4-year-old adult bazhou yaks in Xinjiang region were used as materials. OTU clustering, species abundance and Alpha diversity at phylogenetic and generic levels of rumen bacterial flora of yaks were analyzed by using 16S rRNA gene sequence analysis. At the phylum level, the relative abundance of Firmicutes changed from 66.96% to 70.31%, the relative abundance of Bacteroides changed from 21.87% to 24.11%, and the proportion of other bacterials were very small. At the genus level, the relative abundance of Prevotella in the rumen of young bazhou yaks was higher than that of adult bazhou yaks, the relative abundance of Ruminococcus in the rumen of young bazhou yaks was higher than that of adult bazhou yaks. The Chao1 index of young and adult bazhou yaks were 783.50 and 544.00, Shannon indexes were 4.68 and 4.52, respectively. Therefore, under the same grassland grazing conditions, the rumen bacterial flora composition of 1-year-old and 4-year-old Bazhou yaks was significant difference. The rumen digestion and absorption function of young yaks was in the process of transformation and establishment, so Chao1 and Shannon indexes were higher than that of adult yaks, and the diversity and abundance of rumen bacterial were higher. This results of the study provide important reference for the study of microbial homeostasis and nutritional regulation in yak rumen. |
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| Keywords: | young yak adult yak rumen bacterial diversity 16S rRNA sequence analysis |
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