| 祁白术多酚酶法提取工艺优化及其抗氧化、抑制黑色素合成活性 |
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| 引用本文： | 吴永祥,王雅群,戴毅,金泰完,周讯,陈向阳.祁白术多酚酶法提取工艺优化及其抗氧化、抑制黑色素合成活性[J].核农学报,2019,33(6):1146-1155. |
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| 作者姓名： | 吴永祥 王雅群 戴毅 金泰完 周讯 陈向阳 |
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| 作者单位： | 黄山学院生命与环境科学学院,安徽黄山245041;安东国立大学食品科学与生物技术学院,韩国庆尚北道安东760749;黄山学院生命与环境科学学院,安徽黄山,245041;安东国立大学食品科学与生物技术学院,韩国庆尚北道安东,760749;黄山学院分析测试中心,安徽黄山,245041 |
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| 基金项目： | 安徽省留学回国人员创新项目择优资助计划重点项目(2017srst1),安徽省高校优秀拔尖人才培育项目,安徽省高校自然科学研究重点项目(KJ2017A398),安徽省大学生创新创业训练项目(201810375057、201610375055) |
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| 摘 要： | 为考察祁白术多酚(polyphenols from Atractylodes macrocephala Koidz grown in Qimen, AMP)的可利用性,采用响应面分析法优化酶法提取工艺,并研究AMP的抗氧化、抑制黑色素合成活性。结果表明,在料液比1:30 g·mL-1、酶解时间20 min的条件下,当纤维素酶添加量为1.35%、酶解温度为44℃、pH值为4.7、搅拌转速为670 r·min-1时,AMP提取量最高,为26.58±0.23 mg·g-1。统计学分析显示,所选响应面模型拟合较好,优化后的提取工艺条件合理可行。AMP具有较好的抗氧化活性,其总还原力、对1,1-二苯基-2-三硝基苯肼(DPPH)和2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸)二铵盐(ABTS)自由基清除作用随其浓度的增加而增强。当AMP浓度为0~0.02 mg·mL-1时,对B16细胞无毒性作用。与α-MSH模型组相比,AMP显著下调了细胞内酪氨酸酶活性(P<0.05),并呈浓度依赖性地抑制细胞内黑色素的合成(P<0.05);当AMP浓度为0.02 mg·mL-1时,对细胞内酪氨酸酶活性及黑色素合成抑制率分别为30.11%、43.35%,阳性对照熊果苷(0.1 mg·mL-1)对细胞内酪氨酸酶活性及黑色素合成的抑制率分别为22.03%、39.77%,表明AMP对黑色素生成的抑制效果强于熊果苷。本研究结果为祁白术多酚的综合开发利用提供了依据。
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| 关 键 词： | 祁白术 多酚 响应面分析法 抗氧化 黑色素 |
| 收稿时间： | 2018-10-25 |
Optimization of Enzymatic Extraction and Antioxidant,Anti-melanogenesis Activities of Polyphenols From Atractylodes macrocephala Koidz Grown in Qimen |
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| WU Yongxiang,WANG Yaqun,DAI Yi,KIM Taewan,ZHOU Xun,CHEN Xiangyang.Optimization of Enzymatic Extraction and Antioxidant,Anti-melanogenesis Activities of Polyphenols From Atractylodes macrocephala Koidz Grown in Qimen[J].Acta Agriculturae Nucleatae Sinica,2019,33(6):1146-1155. |
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| Authors: | WU Yongxiang WANG Yaqun DAI Yi KIM Taewan ZHOU Xun CHEN Xiangyang |
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| Institution: | 1 College of Life and Environment Science, Huangshan University, Huangshan, Anhui 245041; 2 Department of Food Science and Biotechnology, Andong National University, Andong, North Gyeongsang Province 760749, Korea; 3 Analysis and Test Center, Huangshan University, Huangshan, Anhui 245041 |
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| Abstract: | In order to investigate the availability of polyphenols from Atractylodes macrocephala Koidz grown in Qimen (AMP), response surface methodology was used to optimize the process parameters for enzymatic extraction technology of AMP. The antioxidant and anti-melanogenesis activities of AMP were explored. The results showed that the optimal process conditions were as follows: solid-liquid ration of 1:30 g·mL-1, enzymatic hydrolysis time of 20 min, cellulase concentration of 1.35%, enzymatic hydrolysis temperature of 44℃, pH value of 4.7, and rotation speed of 670 r·min-1. Under these conditions, the content of AMP was 26.58±0.23 mg·g-1. According to the statistical analysis, the response surface model was confirmed to have a good fitness, and the optimized extraction process was feasible. AMP had strong antioxidant capacities such as total reducing power, 1,1-diphenyl-2- picrylhydrazyl (DPPH) and 2,2’-azinobis-(3-ethylbenzthiazoline-6-sulphonate (ABTS) free radicals scavenging abilities. There was a good linear dependence between the antioxidant capacity and the concentration of AMP. No significant cytotoxicity of AMP was observed at concentrations up to 0.02 mg·mL-1. Compared with α-MSH model, AMP not only significantly down-regulated cell tyrosinase activity (P<0.05), but also dose-dependently inhibited melanin synthesis (P<0.05). The tyrosinase activity and melanin content significantly decreased by 30.11%, 43.35% at 0.02 mg·mL-1 of AMP, respectively. Treatment of 0.1 mg·mL-1 of arbutin reduced the tyrosinase activity and melanin content by 22.03%, 39.77%, respectively. This indicated that AMP showed a stronger anti-melanogenesis activity than that of positive control arbutin. This research could provide useful information for further development and comprehensive utilization of AMP. |
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| Keywords: | Atractylodes macrocephala Koidz grown in Qimen polyphenol response surface methodology antioxidant melanin |
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